Investigations of selected properties of pharmacologically active compounds by chromatographic and potentiometric methods on the chrysin example

• Autorzy: Pusz J. , Zapała W. , Papciak B.
• Języki publikacji: EN

Abstrakty

EN

The RP-TLC method was used to determine the dissociation constant of chrysin (5,7-dihydroxyflavone) in methanol-aqueous (1:1 v/v) solutions. In this method the pK value was quantified on the basis of retention data and a retention model. The accuracy of determining the model parameters was analysed using the following statistical criteria: the sum of the squared differences between the experimental and theoretical data, approximation of standard deviation, and the Fisher test. Besides, in this work the potentiometric method was used. Investigations were carried out at ionic strength I=0.1 at T= 298 K. The dissociation constant were determined using the Rossotti method as well as the numerical method based on the procedures of non-linear curve fitting using Microsoft Excel Solver and the user-defined function. It has been found that the differences in the evaluated pK values were relatively small and did not exceed 1.2%.

Słowa kluczowe

EN

RP-HPLC; retention model; chrysin; dissociation constant

PL

RP-HPLC; model retencji; chryzyna; stała dysocjacji

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2008
• Tom: Vol. 10, nr 1
• Strony: 41--44
• Opis fizyczny: Bibliogr. 18 poz., rys., tab.

Twórcy

autor

Pusz J.

autor

Zapała W.

autor

Papciak B.

• Department of Inorganic and Analytical Chemistry, Rzeszów University of Technology,

Bibliografia

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