The influence of the mobile phase composition on the sorption equilibrium in different HPLC systems

• Autorzy: Zapała W.
• Języki publikacji: EN

Abstrakty

EN

The influence of the mobile phase composition on the equilibrium of the sorption process in different normal and reversed overloaded chromatographic systems has been studied. Two different equations for the description of isotherm parameters of a given solute as a function of the mixed mobile phase composition were proposed. Both models were thoroughly tested in the experiments using many different chromatographic systems. All models were verified by the use of three criteria: the sum of the squared differences between the experimental and theoretical data, approximation of the standard deviation and the Fisher test. All the computation results obtained confirm the fine performance of the proposed equations. The models give good fitting results, accuracy and great applicability. The proposed models can be used for the numerical simulations of the peak profiles and the optimisation of the effects of the mobile phase on the yield, purity and production rate under overloaded isocratic conditions in the preparative liquid chromatography.

Słowa kluczowe

EN

liquid chromatography; mobile phase composition; adsorption isotherm

PL

chromatografia cieczowa; faza ruchoma; izoterma adsorpcji

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2006
• Tom: Vol. 8, nr 3
• Strony: 144--146
• Opis fizyczny: Bibliogr. 6 poz., rys., tab.

Twórcy

autor

Zapała W.

• Department of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, al. Powstańców Warszawy 6, 35-959 Rzeszów,

Bibliografia

• (1) Jandera P., Kucerova M., Holikova J.: Description and prediction of retention in NP-HPLC with binary and ternary mobile phases, J. Chromatogr. A, 1997, 762, 15.
• (2) Zapała W.: Influence of mobile phase composition on retention factors in different HPLC systems with chemically bonded stationary phases, J. Chromatogr. Sci., 2003, 41, 289.
• (3) Zapała W.: Bi-langmuirian retention model in analysis of modifier concentration effects in HPLC retention process, The XXIXth Symposium ,,Chromatographic Methods of Investigating the Organic Compounds", Szczyrk 2005, 70.
• (4) Jandera P., Komers D.: Fitting competitive adsorption isotherm to the experimental distribution data in reversed-phase systems, J. Chromatogr. A, 1997, 762, 3.
• (5) Jandera P., Komers D., Andel L., Prokes L.: Fitting competitive adsorption isotherms to the distribution data in normal phase systems, J. Chromatogr. A, 1999, 831, 131.
• (6) Fletcher R.: A modified Marquardt sub-routine for nonlinear least-squares, Raport AERE-R6799, Harwell, England 1971.