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Tytuł artykułu

Biological degradation of feather keratin by saprophytic bacteria

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In the present study, 30 strains capable of degrading keratin were isolated in selective media containing this protein. A total count of 18 bacterial, 9 fungal and 3 yeast isolates was obtained. The dominating genera were: Bacillus sp., Sarcina sp., Penicillium sp., Fusarium sp., Mucor sp., Geotrichum sp., Rhodotorula sp. and Candida sp. The most effective keratin degradation was performed by the single cultures of bacteria Bacillus and Sarcina as well as Mucor, Penicillium and Fusarium fungi (51÷98%). Feather keratin decomposition by bacterial isolates was analysed in the submerged cultures in the mineral medium, containing keratin as a sole source of carbon and nitrogen. The study shows that the Bacillus polymyxa, B.cereus, B.subtilis and Sarcina sp. bacteria liquified keratin by almost a complete disintegration of its structure, after a few days of incubation. This process was accompanied by the release of soluble proteins (1 mg/cm3), amino groups (210 žg/cm^3) and thiol groups (50 žg/ cm^3). Furthermore, the activity of proteases (48 PU/cm^3 ź min) and keratinases (6 KU/cm3 ź min) in the culture media was observed.
Rocznik
Strony
46--49
Opis fizyczny
Bibliogr. 18 poz., tab., rys.
Twórcy
  • Department of Biotechnology and Food Microbiology, Agricultural University of Wrocław, ul Norwida 25, 50-375 Wrocław
autor
  • Department of Biotechnology and Food Microbiology, Agricultural University of Wrocław, ul Norwida 25, 50-375 Wrocław
Bibliografia
  • (1) Onifade A. A., Al-Sane N. A., Al-Musallam A. A., Zarban S.: A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of fearhers and other keratins as livestock feed resources, Bioresource Technology, 1998, 66, 1.
  • (2) McGovern V.: Recycling poultry feathers: more bang for the cluck, Environmental Health Perspectives, 2000, 108, 8.
  • (3) Shih J. C. H.: Recent development in poultry waste digestion and feather utilization - A review, Poultry Science, 1993, 72, 1617.
  • (4) Wawrzkiewicz K., Łobarzewski J., Wolski T.: Intracellular keratinase of Trichophyton gallinae. J. Medic Veter MycoL, 1987, 25, 261.
  • (5) Snyder S. L., Sobociński P. Z.: An Improved 2,4,6,-trinitrobenzene- sulfonie acid method for the determination of Amines, Analyt. Biochem; 1975, 64, 284.
  • (6) Riener C. R., Kada G., Gruber H. J.: Quick measurment of protein sulfhydryls with Ellman's reagent and with 4,4'-dithiodipyridine, Analyt. Bioanalyt. Chem, 2002, 373, 266.
  • (7) Kunert J.: Biochemical mechanism of keratin degradation by the actinomycete Streptomyces fragies and the fungus of Microsporum gypseum; A comparison, J.Basic. Microbiol, 1989, 29, 597.
  • (8) Sangali S., Brandelli A.: Feather keratin hydrolysis by a Yibrio sp. strain kr2, Journal of Applied Microbiology, 2000, 89, 735.
  • (9) Kunert J.: Effect of reducing agents on proteolytic and keratinolytic activity of enzymes of Microsporum gypseum, Mycoses, 1992, 35, 343.
  • (10) Onifade A. A., Al-Sane N. A., Al-Musallam A. A., Zarban S.: A review: Potentialsfor biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of fearhers and other keratins as livestock feed resources, Bioresource Technology, 1998, 66, 1.
  • (11) Yamamura S., Morita Y., Hasan Q., Rao S.R., Murakami Y., Yokoyama K., Tamiya E.: Characterization of a new keratin-degrading bacterium isolated from deer fur, J. Biosc. Bioengineer., 2002, 93 /6, 595.
  • (12) Ichida J. M., Krizova L., LeFevre C. A., Keener H., Elwell D., Burtt Jr. E.: Bacterial inoculum enhances keratin degradation and biofilm formation in poultry compost, J. Microbiol. Methods, 2001, 47, 199.
  • (13) Kim J. M., Lim W. J., Suh H. J.: Feather-degrading Bacillus species from poultry waste, Process Biochemistry, 2001, 37, 287.
  • (14) Lin X., Inglis G. D., Yanke L. J., Cheng K. J.: Selection and characterization of feather-degrading bacteria from canola meal compost, Journal of Industrial Microbiology and Biotechnology, 1999, 23, 149.
  • (15) Nitisinprasert S., Pornwirun W., Keawsompong S.: Characterizations of two bacterial strains showing high keratinase activities and their synergism in feather degradation, Kasetsart J., 1999, 33, 191.
  • (16) Loo C. Y., Corliss D. A., Ganeshkumar N.: Strepto-coceus gordonii biofilm formation: identification of genes that code for biofilm phenotypes, Journal of Bacteriology, 2000, 182/ 5, 1374.
  • (17) Łaba W., Rodziewicz A.: Biodegradacja odpadów keratynowych z przemysłu drobiowego przy udziale bakterii z rodzajów Bacillus i Sarcina, Acta Sci. Pol. 2004, 3, 75.
  • (18) Rozs M., Manczinger L., Vágvölgyi Cs., Kevei F.: Secretion of a trypsin-like thiol protease by a new keratinolytic strain of Bacillus licheniformis, FEMS Microbiology Letters, 2001, 205, 221.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BPS2-0035-0048
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