Coil-to-helix transition of anionic polysaccharides: comparisond of voltammetric and conductometric results for kappa-carrageenan

• Autorzy: Ciszkowska M. , Guillaume M. D. , Kotlyar I.

Warianty tytułu

PL

Badanie przejścia konformacyjnego spirala-helisa anionowych polisacharydów: porównanie wyników woltamperometrycznych i konduktometrycznych dla kappa-carrageenanu

• Języki publikacji: EN

Abstrakty

EN

Two electroanalytical techniques, steady-state voltammetry and conductance measurements were utilized to study the conformational coil to double helix transition of an ionic polysaccharide /cappa-carrageenan, K-car, in solutions of low ionic strengths as effected by temperature and concentration of the polyion. The interactions betweep counterions, Na+, or electroactive probe ions, Tl+, and polyanions during such transition were quantified by the ratio of the diffusion coefficient of Na+ or Tl+ ions in the polyelectrolyte solution (K-car sodium salt) to that in the solution without polyelectrolyte (NaCl or NaClO4). The results for native counterions, Na+, from conductance measurements were very close to those obtained by voltammetry of Tl+ probe ions. The conformational transition was a reversible coil-to-double helix process resulting from temperature decrease. This process did not depend on the concentration of K-car for the concentration range from 10 to 40 mmol 1-1. The charge spacing in K-car conformers was determined by employing Manning's theory for polyelectrolytes. The average charge spacing calculated from the conductivity and voltammetric results was 0.39 nm and 0.90 nm for the double helix and the coil form of K-car, respectively.

PL

Badano przejście konformacyjne jonowego polisacharydu (kappa-carrageenan, K-car) w funkcji temperatury, mocy jonowej i polijonu używając stacjonarnej woltamperometrii i konduktometrii. Oddziaływanie między polijonem i kontrjonami Na+ oraz sondującymi jonami Ti+ można było określić ilościowo ze zmian współczynników dyfuzji tych jonów. Przejście ze struktury spirali do podwójnej helisy było odwracalne i nie zależało od stężenia K-car w zakresie 10-40 mmol T1. Odległość między ładunkami obliczono używając teorii Manning'a. Otrzymano wielkości 0.39 i 0.90 nm odpowiednio dla form K-car - podwójnej helisy i spirali.

Słowa kluczowe

EN

kappa-carrageenan; coil-to-helix transition; voltammetry; conductometry

PL

kappa-carrageenan; przejście konformacyjne; woltamperometria; konduktometria

• Wydawca: Komitet Chemii Analitycznej PAN
• Czasopismo: Chemia Analityczna
• Rocznik: 2000
• Tom: Vol. 45, No. 2
• Strony: 167--174
• Opis fizyczny: Bibliogr. 22 poz.

Twórcy

autor

Ciszkowska M.

• Department of Chemistry, Brooklyn College, The City University of New York, Brooklyn, NY 11210-2889

autor

Guillaume M. D.

• Department of Chemistry, Brooklyn College, The City University of New York, Brooklyn, NY 11210-2889

autor

Kotlyar I.

• Department of Chemistry, Brooklyn College, The City University of New York, Brooklyn, NY 11210-2889

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