Interaction in mixed amphotericin B / sterols monolayers spread at the air / water interface

• Autorzy: Dynarowicz-Łątka P. , Seoane J. R. , Trillo J. M. , Mouzo O. C. , Parada M. C.
• Języki publikacji: EN

Abstrakty

EN

Different methods for studying molecular interaction between film-forming components in Langmuir monolayers were applied and compared for the mixed systems of amphotericin B with four different sterols: cholesterol, ergosterol, stigmasterol and B-sitosterol. Among the sterols studied, ergosterol exhibits the strongest interaction with amphotericin B and B-sitosterol the weakest. Cholesterol and stigmasterol show interme-diate behaviour. As a consequence of the interactions, deviations from ideality are ob-served; negative at low and positive at high surface pressures. 1'he interactions are most pronounced for monolayers with the mole fraction of amphotericin Xa == 0.5 and 0.7 which lea.ds to the formation of an amphotericin B-sterol complexes of 2:1 stoichiometry. The obtained results support pharmacological assumption that amphotericin B has a greater affinity to ergosterol than to other sterols. Also the presence of amphotericin B-sterol com-plexes of 2: 1 stoichiometry, responsible for the formation of channels in cell membranes, has been confirmed.

Słowa kluczowe

EN

Langmuir monolayers; amphotericin; molecular interaction; air/water interface

• Wydawca: Centrum Upowszechniania Nauki PAN
• Czasopismo: Bulletin of the Polish Academy of Sciences. Chemistry
• Rocznik: 1999
• Tom: Vol. 47, No.2
• Strony: 153--166
• Opis fizyczny: Bibliogr. 48 poz.

Twórcy

autor

Dynarowicz-Łątka P.

autor

Seoane J. R.

autor

Trillo J. M.

autor

Mouzo O. C.

autor

Parada M. C.

• Jagiellonian University, Faculty of Chemistry, Ingardena 3, 30-060 Kraków (Poland)