Zastosowanie metody enzymatycznej do analizy formaldehydu w roztworach wodnych

• Autorzy: Gonchar M.V. , Strzelczyk M. , Maidan M.M. , Bień J. , Sibirny A.A.

Warianty tytułu

EN

Analysis of formaldehyde in aqueous solutions by the enzymatic method

• Języki publikacji: PL

Abstrakty

EN

A new sensitive analytical method was developed.The method enables guantitative analysis of formaldehyde ( up to 0.75 nmol per 1 cm3 reaction mixture) in agueous solutions and municipal sewage.For the purpose of the study,use was made of the non-cancerogenic chromogen,3,3' , 5,5' - tetramenthylobenzidine (TMB),which is oxidized by hydrogen peroxide in the peroxidase reaction to form a dye (which can be determined by photometry).The objective of the present study was to examine the eficiency of the enzymatic kit ALCOTEST ( containing alcohol oxidase,peroxidase,chromogen and buffering components ) when used for the determination of formaldehyde in water solutions and municipal sewage.

Słowa kluczowe

PL

roztwory wodne; analiza formaldehydu

• Wydawca: Polskie Zrzeszenie Inżynierów i Techników Sanitarnych, Oddział Dolnośląski
• Czasopismo: Ochrona Środowiska
• Rocznik: 1998
• Tom: nr 3
• Strony: 35--38
• Opis fizyczny: Bibliogr. 9 poz.,rys.,wykr.

Twórcy

autor

Gonchar M.V.

• Akademia Nauk Ukrainy

autor

Strzelczyk M.

autor

Maidan M.M.

autor

Bień J.

autor

Sibirny A.A.

Bibliografia

• 1. Annual review of the chemical industry, 1989. United Nations, New York 1991.
• 2. D. D. CHIRAS: Environmental science. The Benjamin/Cummings Publ. Co., Redwood City 1991.
• 3. H. REBHEIN etal.: Formaldehyd und Dimethylamin in tiefgekiihlten Fischerzeugnissen aus dem Handel - eine Bestandsaufnahme. Archiv Lebensmittelhyg., 1995, Vol. 46, S. 122-124.
• 4. Y. I. KORPAN, M. V. GONCHAR, A. P. SOLDATKIN, N. F. STARODUB, A. I. SHULGA, A. A. SIBIRNY, A. V. ELSKAYA: Methylotrophic yeast microbiosensor based on ion-selective field effect transistors for methanol and ethanol determination. Anal. Chirn. Acta, 1993, Vol. 271, pp. 203-208.
• 5. Y. I. KORPAN, M. V. GONCHAR, N. F. STARODUB, A. I. SHULGA, A. A. SIBIRNY, A. V. ELSKAYA: A cell biosensor specific for formaldehyde based on pH-sensitive transistors coupled to methylotrophic yeast cells with genetically adjusted metabolism. Anal. Bio- chem., 1993, Vol. 215, pp. 216-222.
• 6. Y. I. KORPAN, M. V. GONCHAR, N. F. STARODUB, A. A. SIBIRNY, A. V. ELSKAYA: Methylotrophic yeast cells as components of biosensors: a formaldehyde analyzer based on a pH-sensitive field effect transistor. Biochemistry (Moscow), 1994, Vol. 59, pp. 141-143.
• 7. M. V. GONCHAR, A. A. SIBIRNY, Y. I. KORPAN, N. F. STARODUB, A. V. ELSKAYA: Methylotrophic yeast cells as a bioactive component for sensor development. I. Biochemistry of formaldehyde-induced acidification of the extracellular medium. Biochemistry (Moscow), 1994, Vol. 59, pp. 721-725.
• 8. M. V. GONCIIAR, A. A. SIBIRNY, M. M. MAIDAN, M. M. POKRASION: Analytical kit for enzymatic determination of alcohol in biological liquids - ALCOTEST. Approved by Health Protection Ministry of Ukraine, 1994.
• 9. M. V. GONCHAR, Y. I. KORPAN, A. A. SIBIRNY: Quantitative photometric analysis of ethanol by means of purified alcohol oxidase and of the mutant cells of methylotrophic yeast. Ukr. Biochem. Zhurn., 1991, Vol. 64, pp. 62-67.