Immobilizacja fizyczna lipaz. Część 1: Immobilizacja lipaz poprzez adsorpcję

• Autorzy: Tarnowska K. , Gruczyńska E. , Kowalski B.

Warianty tytułu

EN

Physical immobilization of lipases. Part 1: Immobilization of lipases by adsorption

• Języki publikacji: PL

Abstrakty

PL

Lipazy stanowią bardzo ważną grupę enzymów o wszechstronnych możliwościach wykorzystania w procesach biotechnologicznych. Preparaty lipaz stosowane są w reakcjach syntezy, hydrolizy i modyfikacji tłuszczów, produkcji związków smakowo-zapachowych, rozdziale mieszanin racemicznych i analityce chemicznej. W artykule przedstawiono problematykę immobilizacji lipaz przez adsorpcję oraz dokonano przeglądu handlowych nośników stosowanych do adsorpcji enzymów lipolitycznych. Szczególną uwagę poświęcono zagadnieniu selektywnej adsorpcji lipaz na nośnikach o silnie hydrofobowej powierzchni.

EN

Lipases are one of the important enzymes, which are used in specific organie synthesis, hydrolysis of fats and oils, modification of fats, flavor enhancement in food processing, resolution of racemic mixtures and chemical analyses. This article contains information concerning the immobilization of lipases by adsorption and characteristic of commercial carriers used for this purpose. Special emphasis is paid to the selective adsorption of lipases on strongly hydrophobic support surfaces.

• Wydawca: Wyższa Szkoła Menadżerska
• Czasopismo: Postępy Techniki Przetwórstwa Spożywczego
• Rocznik: 2008
• Tom: nr 2
• Strony: 85--91
• Opis fizyczny: Bibliogr. 55 poz., rys., tab.

Twórcy

autor

Tarnowska K.

autor

Gruczyńska E.

autor

Kowalski B.

• SGGW w Warszawie, Wydział Nauk o Żywności

Bibliografia

• [1] Adamczak M., Bednarski W.: Enhanced activity of in-tracellular lipases from Rhizomucor miehei and Yar-rowia lipolytica by immobilization on biomass support particles, Process Biochem., 2004, 39, 1347-1361.
• [2] Al.-Duri B., Yong Y.P.: Lipase immobilization: an equ-librium study of lipases immobilized on hydrophobic and hydrophilic/hydrophobic supports, Biochem. Eng. J., 2000, 4,207-215.
• [3] Antczak T., Graczyk J., Szczęsna -Antczak M., Bielecki S.: Activation of Mucor circinelloides lipase in organie medium, J. Mol. Catal. B: Enzymatic, 2002, 19-20,287-294.
• [4] Antczak T., Graczyk J.: Lipazy: źródła, struktura i właściwości katalityczne, Biotechnologia, 2002, 2 (57), 130-145.
• [5] Arica M.Y., Kacar Y, Ergene A., Denizli A.: Reversible immobilization of lipase on phenylalanine containing hydrogel membranes, Process Biochem., 2001, 36, 847-854.
• [6] Bastida A., Sabuąuillo P., Armisen P, Fernandez-Lafuente R., Huguet J., Giusan J.M.: A Single Step Purifi-cation, Immobilization, and Hyperactivation of Lipases via Interfacial Adsorption on Strongly Hydrophobic Supports, Biotechnol. Bioeng., 1998, 58, 5, 486-493.
• [7] Bellezza F., Cipiciani A., Costantino U.: Esterase activ-ity of biocomposites constituted by lipase adsorbed on layered zirconium phosphate and phosphonates: selec-tive adsorption of different enzyme isoforms, J. Mol. Catal. B: Enzymatic, 2003, 26, 47-56.
• [8] Blanco R.M., Terreros P, Fernandez-Perez M., Otero C, Diaz-Gonzalez G.: Functionalization of mesoporous silica for lipase immobilization, Characterization of the support and the catalysts, J. Mol. Catal. B: Enzymatic, 2004, 30, 83-93.
• [9] Bryjak J., Trochimczuk A. W.: Immobilization of lipase and penicillin acylase on hydrophobic acrylic carriers, Enzyme Microb. Technol., 2006, 39, 4, 573-578.
• [10] Brzozowski A.M., Derewenda U., Derewenda Z.S., Dodson G.G., Lawson D.M., Turkenburg J.P., Bjorkling F., Huge - Jensen B., Patkar SA, Thim L.: A model for interfacial activation in lipases from the structure of a fungal lipase - inhibitor complex, Nature, 1991, 351, 491-494.
• [11] Camacho F., Robles A., Gonzalez PA., Camacho B., Es-teban L., Molina E.: Mechanistic model for the lipase - catalyzed alcoholysis of triacylglycerols, App. Catal. A; General, 2006, 301, 2, 158-168.
• [12] Dalia-Vecchia R., Sebrao D., Nascimento M.G., Soldi V.: Carboxymethylcellulose and poły (vinyl alcohol) used as a film support for lipases immobilization, Process Biochem., 2005, 40, 8, 2677-2682.
• [13] de Fuentes I.E., Viseras CA., Ubiali D., Terreni M., Al-cantara A.R.: Different phyllosilicates as supports for lipase immobilization, J. Mol. Catal. B: Enzymatic, 2001, 11,657-663.
• [14] Deng H.-T., Xu Z.-K., Liu Z.-M., Wu J., Ye R: Adsorption immobilization of Candida rugosa lipases on poly-propylene hollow fiber microfiltration membranes modi-fied by hydrophobic polypeptides, Enzyme Microb. Technol., 2004, 35, 5, 437-443.
• [15] Dumitriu E., Secundo F., Patarin J., Fechete I.: Prepara-tion and properties of lipase immobilized on MCM-36 support, J. Mol. Catal. B: Enzymatic, 2003,22, 119-133.
• [16] Fernandez-Lafuente R., Armisen P, Sabuquillo P, Fernandez-Lorente G, Guisan J.M.: Immobilization of lipases by selective adsorption on hydrophobic supports, Chem. Phys. Lipids, 1998, 93, 185-197.
• [17] Fernandez-Lorente G., Terreni M., Mateo C, Bastida A., Fernandez-Lafuente R., Dalmases P, Huguet J., Guisan J.M.: Modulation of lipase properties in macro-aąueous systems by controlled enzyme immobilization: enan-tioselective hydrolysis of chiral ester by immobilized Pseudomonas lipase, Enzyme Microb. Technol., 2001, 28, 389-396.
• [18] Gandhi N.N., Vijayalakshmi V., Sawant S.B., Joshi J.B.: Immobilization of Mucor miehei lipase on ion exchange resins, Chem. Eng. J., 1996, 61, 149-156.
• [19] Hernandez-Martin E., Otero C: Different enzyme requirements for the synthesis of biodiesel: Novozym (R) 435 and Lipozyme (R) TL IM, Bioresour. Technol, 2007, 99, 2, 277-286.
• [20] Hossen M., Hernandez E.: Enzyme - catalyzed synthesis of structured phospholipids with conjugated linoleic acid, Eur. J. Lipid Sci. Technol., 2005, 107, 730-736.
• [21] Ivanov A.E., Schneider M.P.: Methods for the immobilization of lipases and their use for ester synthesis, J. Mol. Catal. B: Enzymatic, 1997, 3, 303-309.
• [22] Jennings B.H., Akoh CC: Lipase - catalyzed modifica-tion of fish oil to incorporate capric acid, Food Chemis-try, 2001, 72, 273-278.
• [23] Jonzo M.D., Hiol A., Zagoi L, Druet D., Comeau L.-C: Concentrates of DHA from fish oil by selective esterifi-cation of cholesterol by immobilized isoforms of lipase from Candida rugosa, Enzyme Microb. Technol., 2000, 27, 443-450.
• [24] Kadam M., Bhowmick D.N.: Lipase - catalyzed modi-fication of black currant oil, J. Food Lipids, 2006, 13,2, 167-176.
• [25] Kamori M., Hori T, Yamashita Y, Hirose Y, Naoshima Y: Immobilization of lipase on a new inorganic ceram-ics support, toyonite, and reactivity and enatioselectivity of the immobilized lipase, J. Mol. Catal. B: Enzymatic, 2000, 9, 269-274.
• [26] Khare S.K., Nakajima M.: Immobilization of Rhizopus japonicus lipase on celite and its application for enrich-ment of docosahexaenoic acid in soybean oil, Food Chemistry, 2000, 68, 153-157.
• [27] Kowalak S., Stawiński K.: Mezoporowate sita molekularne, otrzymywanie i właściwości, Wiadomości Chemiczne, 2000, 54, 9-10, 817-843.
• [28] Kowalski B., Tarnowska K., Gruczyńska E.: Enzymatic acidolysis of beef tallow with lauric acid, Riv. Ital. So-stanze Grasse, 2004, LXXXI, 284-289.
• [29] Kowalski B., Tarnowska K., Gruczyńska E., Bekas W.: Chemical and enzymatic interesterification of a beef tallow and rapeseed oil eąual-weight blend, Eur. J. Lipid Sci. Technol., 2004, 106, 655-664.
• [30] Lee Ch.-H., Parkin K.L.: Effect of Water Acivity and Immobilization on Fatty Acid Selectivity for Esterifica-tion Reactions Mediated by Lipases, Biotechnol. Bioeng., 2001, 75, 2, 219-227.
• [31] Liu Y-Y, Xu J.-H., Wu H.-Y, Shen D.: Integration of purification with immobilization of Candida rugosa lipase for kinetic resolution of racemic ketoprofen, J. Biotechnol., 2004, 110,209-217.
• [32] Ma H., He J., Evans D.G., Duan X.: Immobilization of lipase in a mesoporous reactor based on MCM-41, J. Mol. Catal. B: Enzymatic, 2004, 30, 5-6, 209-217.
• [33] Miller C, Austin H., Posorske L., Gonzalez J.: Charac-teristics of an immobilized lipase for the commercial synthesis of esters, JAOCS, 1988, 65, 6, 927-931.
• [34] Murray M., Rooney D., Van Neikerk M., Montenegro A., Weatherley L.R.: Immobilization of lipase onto lipo-philic polymer particles and application to oil hydroly-sis, Process Biochem., 1997, 32, 6, 479-486.
• [35] Nieto I., Rocchietti S., Ubiali D., Speranza G., Morelli CR, Fuentes I.E., Alcantara A.R., Terreni M.: Immobilization of different protein fraction from Rhizomucor miehei lipase crude extract, Enzymatic resolution of (R, 5>2-tetralol, Enzyme Microb. Technol., 2005, 37, 514-520.
• [36] 0'Connell RJ., Varley J.: Immobilization of Candida rugosa Lipase on Colloidal Gas Aphrons (CGAs), Bio-technol. Bioeng., 2001, 74, 3, 264-269.
• [37] Otero C, Femandez - Perez M, Perez - Gil J.: Effect of interactions of micellar interfaces on the activity and structure of different lipolytic isoenzymes from Candida rugosa, Enzyme Microb. Technol., 2005, 37, 695-703.
• [38] Palomo J.M., Fernandez-Lorente G., Mateo C, Ortiz C., Fernandez-Lafuente R., Guisan J.M.: Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters, Enzyme Microb. Technol., 2002, 31,775-783.
• [39] Palomo J.M., Muńoz G., Fernandez-Lorente G., Mateo C, Fernandez-Lafuente R., Guisan J.M.: Interfacial adsorption of lipase on very hydrophobic support (octa-decyl-Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases, J. Mol. Catal. B: Enzymatic, 2002, 19-20, 279-286.
• [40] Persson M., Mladenoska I., Wethje E., Adlercreutz P: Preparation of lipases for use in organie solvents, Enzyme Microb. Technol., 2002, 31,6, 833-841.
• [41] Pu W., Li-rong Y., Jian-ping W.: Immobilization of lipase by salts and transesterification activity in hexane, Biotechnol. Lett, 2001, 23, 1429-1433.
• [42] Rahman M.B.A., Tajudin S.M., Hussein M.Z., R.N.Z.R.A. Rahman Salleh A.B., Basri M.: Application of natural kaolin as support for the immobilization of lipase from Candida rugosa as biocatalyst for effective esterification, Appl. Clay Sci., 2005, 29, 2, 111-116.
• [43] Romero M.D., Calvo L., Alba C, Habulin M., Primo-zic M., Knez Z.: Enzymatic synthesis of isoamyl acetate with immobilized Candida antaretica lipase in supereri-tical carbon dioxide, J. Suprecritical Fluids, 2005, 33, 1, 77-84.
• [44] Rosu R., Iwasaki Y., Shimizu N., Doisaki N., Yamane T: Intensification of lipase performance in a transesterification reaction by immobilization on CaCO^ powder, J. Biotechnol., 1998,66,51-59.
• [45] Salis A., Sanjust E., Solinas V., Monduzzi M.: Charac-terisation of Accurel MP1004 polypropylene powder and its use as a support for lipase immobilization, J. Mol. Catal. B: Enzymatic, 2003, 24-25, 75-82.
• [46] Sarda L., Desnuelle P: Action de la lipase pancreatiaue sur les esters en emulsion, Biochim. Biophys.Acta, 1958, 30,513-521.
• [47] Senanayake S.P.J.N., Shahidi F: Lipase - catalyzed in-corporation of docosahexaenoic acid (DHA) into borage oil: optimization using surface methodology, Food Chemistry, 2002, 77, 115-123.
• [48] Stadler P, Kovac A., Paltauf F: Understanding lipase action and selectivity, Croatia Chemica Acta, 1995, 68, 3, 649-674.
• [49] Talukder Md.M.R., Tamalampudy S., Li Ch.J., Yanglin L., Wu J., Kondo A., Fakuda H.: An improved method of lipase preparation incorporating both solvent treat-ment and immobilization onto matrix, Biochem. Eng. J., 2007,33, 1,60-65.
• [50] Tarnowska K., Gruczyńska E., Kowalski B.: Immobi-lizacja kowalencyjna lipaz, Postępy Techniki Przetwórstwa Spożywczego, 2008, 1, 72-78.
• [51] Trusek - Holownia A., Noworyta A.: Catalytic membrane preparation for enzymatic hydrolysis reaction car-ried out in the membrane phase contactor, Desalination, 2002, 144,427-432.
• [52] Tynek M., Ledóchowska E.: Structured triacylglycerols containing behenic acid: preparation and properties, J. Food Lipids, 2005, 12, 1, 77-89.
• [53] Van Tilbeurgh H., Egloff M.P., Martinez C, Rugani N., Verger R., Cambillau C: Interfacial activation of lipase-procolipase complex by mixed micelles revealed X-ray crystallography, Namre, 1993, 362, 814-820.
• [54] Xu D., Li Z., Ma S.: Novozym-435-catalyzed enzymatic separation of racemic propargylic alcohols, A facile route to optically active terminal aryl propargylic alcohols, Tetrahedron Lett., 2003, 44, 33, 6343-6346.
• [55] Yesiloglu Y: Utilization of bentonite as a support materiał for immobilization of Candida rugosa lipase, Process Biochem., 2005, 40, 6, 2155-2159.