Impact of bioreactor scale on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture

• Autorzy: Pawlak M. , Bizukojć M. , Ledakowicz S.
• Języki publikacji: PL

Abstrakty

EN

Biosynthesis of lovastatin (a polyketide metabolite of Aspergillus terreus) in bioreactors of different working volume was studied to indicate how the change of scale of the process influences the formation of this metabolite. The experiments conducted in shake flasks of 150 ml working volume allowed to obtain lovastatin titres at the level of 87.5 mg LOV l-1, when two carbon sources, namely lactose and glycerol were used. The application of the same components in a large stirred-tank bioreactor of 5.3-litre working volume caused a decrease of lovastatin production by 87% compared to the shake flask culture. The deficiency of nitrogen in this bioreactor did not favour the formation of lovastatin, in contrast to the small bioreactor of 1.95-litre working volume, in which lovastatin titres comparable to those in the shake flasks could be achieved, when organic nitrogen concentration was two-fold decreased. When the control of pH and/or pO2 was used simultaneously, an increase in lovastatin production was observed in the bioreactors. However, these results were still slightly lower than lovastatin titres obtained in the shake flasks.

Słowa kluczowe

PL

lowastatyna; bioreaktor; proces wsadowy

EN

lovastatin; Aspergillus terreus ATCC 20542; shake flask; stirred tank bioreactor; batch process

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering
• Rocznik: 2012
• Tom: Vol. 33, nr 1
• Strony: 71--84
• Opis fizyczny: Bibliogr. 16 poz., tab., wykr.

Twórcy

autor

Pawlak M.

autor

Bizukojć M.

autor

Ledakowicz S.

• Technical University of Lodz, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, ul. Wólczańska 213, 90-924 Łódź, Poland

Bibliografia

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