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Functional cardio-biomaterials

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Titanium as well as carbon based biomaterials, seem to be good candidates for future blood-contact applications. Bio-materials such as: Ti, Ti+DLC, TiN; Ti(C,N) with higher carbon concentration and DLC (diamond like carbon) were under examinations. Trials on surface modification of PU (polyurethane) substrate using ion mill were performed. Materials were deposited as thin films by the hybrid pulsed laser deposition (PLD) technique to examine the influence of the fabricated surfaces on cell behavior. The metallic titanium as a target was used for titanium based-film and graphite one for DLC. Phase content of deposited films was controlled by the flowing gas mixture of Ar+N2 and Ar+N2+C2H2 type in the reactive chamber. Sputtering of graphite was carried out in Ar atmosphere. The kinetic energy of the evaporated particles was controlled by application of variation of different reactive and non reactive atmospheres during deposition. Transmission electron microscopy (TEM) was used to reveal structure dependence on specific atmosphere in the reactive chamber. The measurement of the strength of bonds between biomaterials and cells is a major challenge in cellular biology since it allows for the identification of different species in adhesion phenomena. The biomaterial examinations were performed in static conditions with Dictyostelium discoideum cells and then subjected to a dynamical test to observe the cell detachment kinetics. For a given cell, detachment occurs for critical stress values caused by the applied hydrodynamic pressure above a threshold which depends on cell size and physicochemical properties of the substrate, but it is not affected by depolymerization of the actin and tubulin cytoskeleton. Tests revealed differences in behavior in respect to the applied coating material. The strongest cell-biomaterial interaction was observed for the carbon-based materials compared to the titanium and titanium nitride. A surface fuctionalization was realized by: (i) fabrication of migration channels by laser ablation, (ii) electrospinning and (iii) deposition of multilayer film from polyelectrolites. A goal of the performed research was formation of scaffolds for bio-mimetic coatings. Surface morphology examinations and biomedical studies on porous and semi-porous materials with application of human endothelial cells HUVEC line were performed by application of confocal laser scanning microscopy (CLSM).
Rocznik
Strony
5--25
Opis fizyczny
Bibliogr. 34 poz., rys., tab.
Twórcy
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Bibliografia
  • 1. Ratner B. D, Hoffman A. S, Schoen F. J, Lemons J. E. Biomaterials Science, Copyright Elsevier Inc. 2004.
  • 2. Bozzaro S and Ponte E. Cell adhesion in the life cycle of Dictyostelium, Experientia; 51 (1995) 1175-81.
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  • 4. Morio T, Urushihara H, Saito T, Ugawa Y, Mizuno H, Yoshida M, Yoshino R, Mitra B.N, Pi M, .Sato T, Takemoto K, Yasukawa H, Williams J, Maeda M. Takeuchi I, Ochiai H and Tanaka Y. The Dictyostelium discoideum developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development. DNA Res. 5 (1998) 335.
  • 5. E.Decave, Y.Brechet, F.Bruckert, and B.Fourcade, “Peeling model for cell detachment D. Garrivier”, Eur. Phys. J., E 8, (2002)79–97.
  • 6. Décavé E, Garrivier D, Bréchet Y, Fourcade B and Bruckert F. Shear Flow-Induced Detachment Kinetics of Dictyostelium discoideum Cells from Solid Substrate Biophysical Journal. 82 (2002) 2383–2395.
  • 7. Bruckert F, De Cave E, Garrivier D, Cossion P, Brechet Y, Fourcade B and Satre M. Dictyostelium discoideum adhesion and motility under shear flow: experimental and theoretical approaches. Journal of Muscle Research and Cell Motility, Kluwer Academic Publishers, Netherlands. 23(2002) 651–658.
  • 8. Bruckert F, Demily M, Brecht Y, Boulangé L. Kinetics of yeast detachment from controlled stainless steel surfaces; Colloids and Surfaces B, Biointerfaces, Elsevier Inc. 51(2006) 71-79.
  • 9. Bäuerle D. Laser Processing and Chemistry, Springer–Verlag, Berlin, Heidelberg 2000.
  • 10. Lackner J.M, Waldhauser W, Schwarz M, Mahoney L, Major L, Major B. Polymer pre-treatment by linear anode layer source plasma for adhesion improvement of sputtered TiN coatings. Vacuum 83(2009) 302-307.
  • 11. Lackner J.M. Industrially- scaled hybrid Pulsed Laser Deposition at Room Temperature. Published by Orekop sc., Krakow, 2005.
  • 12. Major B, Bruckert F, Lackner J.M, Ebner R, Kustosz R, Lacki P. Coating on TiN and Ti(C,N) basis for biomedical application to blood contact and TiN/CrN multilayered tribological systems produced by pulsed laser deposition. Arch.Metal.and Mater. 53 (2008) 39-48.
  • 13. Lackner J.M, Waldhauser W, Alamanou A, Teicher C, Schmied F, Major L, Major B. Mechanisms for self-assembling topography formation in low-temperature vacuum deposition of inorganic coatings on polymer surfaces. Bull.Pol.Ac.:Tech. 58(2) (2010) 281-294.
  • 14. Sarna J, Kustosz R, Major R, Lackner J.M, Major B. Polish Artificial Heart – new coatings, technology, diagnostics. Bull.Pol.Ac.Tech. 58(2) (2010) 329-336.
  • 15. Major R, F.Bruckert F, J.M.Lackner J.M, Waldhauser W, Pietrzyk M, Major B. Kinetics of eucariote cells adhesion under shear flow detachment on the PLD deposited surfaces. Bull.Pol.Ac.Ser.Techn. 56(2008) 223-228.
  • 16. Sanak M, Jakiela B, Wegrzyn W. Assesment of hemocompatibility of materiale with arteria blond flow by platelet functional tests. Bull.Pol.Ac.Tech. 58(2) (2010) 317-322.
  • 17. Thornton J.A, Influence of apparatus geometry and deposition conditions on the structure and topography of thick sputtered coatings. J.Vac.Sci.Technol., 11(4) (1974) 666-670.
  • 18. WinSAM Vario I to III, and SAM 2000, KSI, Kraemer Scientific Instruments, Lerchenweg 16-18, 35745 Herborn, Germany.
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  • 20. Acoustic microscopy, Andrew Briggs (Monographs on the physics and chemistry of materials, Vol. 46), Oxford University Press, 1992.
  • 21. EVOLUTION I-III, SAM TEC GmbH, P.O. 3111, 73641 Aalen, Email: info@samtec-germany.com.
  • 22. Seyfert U.T, Biehl V and Schenk J. In vitro hemocompatibility testing of biomaterials according to the ISO 10993-4. Biomol. Eng. 19(2-6) (2002) 91-96.
  • 23. M. Otto M, Klein C.L, Koehler H, Wagner M, Roehrig O and Kirkpatrick C.J. Dymanic blood cell contact with biomaterials: validation of a flow chamber system according to international standards. J Mater Sci Mater Med., 8(3)(1997)119-129.
  • 24. Streller U, Sperling C, Huebner J, Ranke R and Werner C. Design and evaluation of novel blood incubation systems for in vitro hemocompatibility assessment of planar solid surfaces. J. Biomed. Mater. Res. B Appl. Biomater. 66B (1) (2003) 379-390.
  • 25. Schaub R.D, Kameneva M.V, Borovetz H.S and W.R. Wagner W.R. Assessing acute platelet adhesion on opaque metallic and polymeric biomaterials with fiber optic microscopy. J. Biomed. Mater. Res. 49 (4) (2000) 460-468.
  • 26. Sbrana S, Della Pina F, Rizza A, Buffa M, De Filippis R, Gianetti J and Clerico A. Relationships between optical aggregometry (type born) and flow cytometry in evaluating ADP-induced platelet activation. Cytometry B Clin Cytom. 74 (1)(2008)30-39.
  • 27. Nomura S, Shouzu A, Tamoto K, Togane Y, Goto S, Uchiyama S and Ikeda Y. Assessment of an ELISA kit for platelet-derived microparticles by joint research at many institutes in Japan. J. Atheroscler. Thromb. JOI JST.JSTAGE/jat/26432. 2009, published online.
  • 28. Harrison P. Progress in the assessment of platelet function. Br J Hematol. 111(3) (2000) 733-744.
  • 29. Shenkman B., Savion N, Dardik R, Tamarin I and Varon D. Testing of platelet deposition on polystyrene surface under plow conditions by the cone and plate(let) analyser: role of platelet activation, fibrinogen and von Willebrand factor. Thromb. Res. 99 (4) (2000) 353-362.
  • 30. Gemmel C.H. Activation of platelets by in vitro whole blood contact with materials: increases in microparticle procoagulant activity, and soluble P-selectin blood levels. J. Biomater. Sci. Polym. Ed. 12 (8) (2001) 933-934.
  • 31. Varon D, Lashevski I, Brenner B, Beyar R, Lanir N, Tamarin I and Savion N. Cone and plate(let) analyzer: monitoring glycoprotein IIb/IIIa antagonists and von Willebrand disease replacement therapy by testing platelet deposition under flow conditions. Am Heart J. 135 (5 Pt 2 Su)S(1998) 187-193.
  • 32. Nomura S, Ozaki Y and Ikeda Y. Function and role of microparticles in various clinical settings” Thromb. Res. 123 (1) (2008) 8-23.
  • 33. Blann A, Shantsila E and Shantsila A. Microparticles and arterial disease. Semin. Thromb. Hemost. 35 (5) (2009) 488-496.
  • 34. Gemmel C.H, Ramirez S.M, Yeo E.L and Sefton M.V. Platelet activation in whole blood by artificial surfaces: identification of platelet-derived microparticles and activated platelet binding to leukocytes as material-induced activation events. J. Lab. Clin. Med. 125 (2) (1995) 276-287.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BPG8-0049-0026
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