Immobilizowane enzymy w bioremediacji środowisk zanieczyszczonych arenami

Wojcieszyńska, D.; Guzik, U.; Nowak, A.; Hupert-Kocurek, K.

Artykuł - szczegóły

Tytuł artykułu

Immobilizowane enzymy w bioremediacji środowisk zanieczyszczonych arenami

Autorzy

Wojcieszyńska D. , Guzik U. , Nowak A. , Hupert-Kocurek K.

Identyfikatory

Warianty tytułu

Immobilized enzymes in bioremediation of arene polluted environments

Języki publikacji

Abstrakty

Związki aromatyczne, stanowiące duże zagrożenie dla organizmów żywych, rozkładane są przez mikroorganizmy do czterech głównych intermediatów: katecholu, kwasu protokatechowego, hydroksychinolu i kwasu gentyzynowego. Ze względu na dużą wrażliwość wolnych enzymów na zmienne warunki środowiska coraz częściej w technologiach oczyszczania ścieków stosuje się immobilizowane enzymy. Obecnie najpowszechniej stosowanym związkiem sieciującym jest glutaraldehyd, a nośnikami - membrany w układach przepływowych. Spośród enzymów stosowanych w technologiach oczyszczania terenów zdegradowanych, miedzy innymi zastosowanie znalazły immobilizowane: peroksydaza, lakaza oraz dioksygenazy katecholowe. Zaimmobilizowane enzymy odznaczają się większą stabilnością w czasie oraz odpornością na zmienne warunki środowiska. Ponadto stosowanie immobilizowanych enzymów pozwala na ich odzyskiwanie po zakończonym procesie, co obniża koszty bioprocesów.

Aromatic compounds are considered as one of the most toxic and weakly degraded xenobiotics. They are degradated to four key intermediates such as catechol, protocatechuate, hydroxyquinol and gallic acid. Free enzymes are very sensitive to changing conditions of environment. That is a reason of immobilized enzymes very often use in technologies of sewage treatment. Glutaraldehyde is the most popular cross-linking agent and membranes in flow systems are the most often applied carrier in immobilization at present. The most often using enzymes in environmental treatment are immobilized peroxidise, laccase and catechol dioxygenases. Immobilized enzymes are more stable in time and resistant to environmental conditions changes. Immobilized enzymes use allow to recover them after finished process and, in the result, bioprocess cost reduction.

Słowa kluczowe

enzymy immobilizacja mikroorganizmy związki aromatyczne degradacja

enzymes immobilization microorganisms aromatic compounds degradation

Wydawca

Bydgoskie Towarzystwo Naukowe

Czasopismo

Ekologia i Technika

Rocznik

2011

Tom

R. 19, nr 2

Strony

53--61

Opis fizyczny

Bibliogr. 50 poz., rys., tab.

Twórcy

autor

Wojcieszyńska D.

autor

Guzik U.

autor

Nowak A.

autor

Hupert-Kocurek K.

Uniwersytet Śląski, Wydział Biologii i Ochrony Środowiska, Katedra Biochemii, 40-032 Katowice, ul. Jagiellońska 28

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