Hyphenated and unconventional methods for searching volatile cancer biomarkers

• Autorzy: Ulanowska A. , Ligor T. , Michel M. , Buszewski B.

Warianty tytułu

PL

Łączone i niekonwencjonalne metody poszukiwania lotnych biomarkerów chorób nowotworowych

• Języki publikacji: EN

Abstrakty

EN

Volatile organic compounds produced inside the body provide valuable information about human state of health and they are detected in breath, blood and urine samples. Therefore, volatile biomarker analysis seems to become accurate and fast method for tumour detection. So far, there are known several volatile organic compounds (VOCs) recognized as potential cancer biomarkers. For the detection of VOCs different analytical techniques are used. The most popular is gas chromatography coupled with mass spectrometry (GC/MS). More recently, selected ion flow tube mass spectrometry (SIFT-MS), proton transfer reaction mass spectrometry (PTR-MS) and ion mobility spectrometry (IMS) are also applied for biomarker research. Besides typical instrumental methods used for VOCs analysis, unconventional methods such as sensitive canine sense of smell can be used. In recent years, this very sensitive scent is also used for cancer biomarker detection. Dogs are trained to recognize the smell of skin, breath or urine samples from patient with different kind of cancer from the control group. The application of dogs’ smell for the preliminary screening of tumour in human body is painless, noninvasive and fast method. Additionally, it does not need the preconcentration of analytes before the analysis.

PL

Lotne związki organiczne (VOCs), powstające wewnątrz organizmu ludzkiego dostarczają wielu cennych informacji na temat stanu zdrowia pacjenta i są one identyfikowane w probkach powietrza wydychanego, krwi i moczu. Wiedzę na ten temat wykorzystuje się podczas analizy lotnych biomarkerów, ktora w przyszłości może zaowocowa. opracowaniem nieinwazyjnej metody diagnostyki medycznej stosowanej do wczesnego wykrywania nowotworow. Obecnie znanych jest kilkadziesiąt związkow organicznych, ktore uznane zostały za potencjalne biomarkery chorób nowotworowych. Substancje lotne są analizowane rożnymi dostępnymi technikami analitycznymi. Wśród nich najbardziej znana jest chromatografia gazowa sprzężona ze spektrometrii mas (GC/MS). Jednakże do oznaczania biomarkerow wykorzystywana jest również spektrometria mas z jonizacją w strumieniu wybranych jonów (SIFT-MS), spektrometria mas z reakcją przeniesienia protonu (PTR-MS) oraz spektrometria ruchliwości jonów (IMS). Techniki te umożliwiają bezpośrednią analizę np. powietrza wydychanego, która prowadzona jest w czasie rzeczywistym. Oprocz tradycyjnych metod instrumentalnych stosowanych do wykrywania biomarkerow wykorzystywana jest rownież dość niekonwencjonalna metoda korzystująca z niezwykle czułego powonienia psów. Tresowane psy są w stanie niemal bezbłędnie odróżnić po zapachu probkę moczu, oddechu oraz skory objętej czerniakiem, pochodzącą od pacjenta i od osoby zdrowej. Technika ta jest bezbolesna, nieinwazyjna oraz szybka. Ponadto próbki do analizy nie muszą być wcześniej wzbogacone.

Słowa kluczowe

EN

analytical methods; canine scent; biomarkers; volatile organic compounds; cancer

PL

metody analityczne; węch psów; biomarkery; lotne związki organiczne; nowotwór

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. S
• Rocznik: 2010
• Tom: Vol. 17, nr 1
• Strony: 9--23
• Opis fizyczny: Bibliogr. 65 poz., rys., tab.

Twórcy

autor

Ulanowska A.

autor

Ligor T.

autor

Michel M.

autor

Buszewski B.

• Katedra Chemii Środowiska i Bioanalityki, Wydział Chemii, Uniwersytet Mikołaja Kopernika

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