Effect of chitosan and Zaprawa Oxafun T on the healthiness and communities of rhizosphere microorganisms of runner bean (Phaseolus coccineus L.)

• Autorzy: Patkowska E.

Warianty tytułu

PL

Wpływ chitozanu i Zaprawy Oxafun T na zdrowotność i zbiorowiska mikroorganizmów ryzosferowych fasoli wielokwiatowej (Phaseolus coccineus L.)

• Języki publikacji: EN

Abstrakty

EN

Studies are presented concerning the impact of pre-sowing seed dressing with biopreparation Biochikol 020 PC and a chemical preparation Zaprawa Oxafun T on the healthiness of Phaseolus coccineus and on the formation of microorganisms in the rhizosphere of this plant. The protective effect of the preparations was high since significantly more plants grew from the dressed seeds, with a smaller proportion of plants with disease symptoms as compared with the seeds that were not dressed (control). However, slightly higher effectiveness in the protection of runner bean was found out after the application of the biopreparation. The following fungi were mainly isolated from the infected parts and the rhizosphere of Phaseolus coccineus: Alternaria alternata, Botrytis cinerea, Fusarium spp., Gliocladium spp., Penicillium spp., Phoma spp., Pythium irregulare, Rhizoctonia solani, Sclerotinia sclerotiorum and Trichoderma spp. Both Biochikol 020 PC and Zaprawa Oxafun T bad a positive effect on the communities of bacteria and fungi in the rhizosphere of runner bean. The total population of bacteria, the populations of Bacillus spp. and Pseudomonas spp. in 1 g of d.m. of the soil after the application of these preparations were significantly greater than in the control. A reverse relation occurred in the case of fungi population. The most of antagonistic bacteria (Bacillus sp., Pseudomonas sp.) and fungi (Gliocladium sp., Trichoderma sp.) in bean rhizosphere were obtained while dressing the seeds with Biochikol 020 PC.

PL

Przedstawiono badania dotyczące przedsiewnego zaprawiania nasion biopreparatem Biochikol 020 PC i preparatem chemicznym Zaprawa Oxafun T na zdrowotność Phaseolus coccineus oraz na kształtowanie się mikroorganizmów w ryzosferze tej rośliny. Efektywność ochronnego działania preparatów była duża, bowiem z nasion zaprawionych wyrosło znacznie więcej roślin, z mniejszym udziałem roślin mających objawy chorobowe aniżeli z nasion niezaprawionych (kontroli). Nieco większą skuteczność w ochronie fasoli stwierdzono jednak po zastosowaniu biopreparatu. Z porażonych organów oraz ryzosfery P. coccineus izolowano głównie takie grzyby, jak: Altemaria alternata. Botrytis cinerea, Fusarium spp., Gliocladium spp., Penicillium spp., Phoma spp., Pythium irregulare. Rhizoctonia solani, Sclerotinia sclerotiorum i Trichoderma spp. Zarówno Biochikol 020 PC, jak i Zaprawa Oxafun T wpłynęły korzystnie na zbiorowiska bakterii i grzybów w ryzosferze fasoli. Ogólna liczebność bakterii, liczebność Bacillus spp. i Pseudomonas spp. w 1 g s.m. gleby po zastosowaniu tych preparatów była znacznie większa aniżeli w kombinacji kontrolnej. Odwrotna zależność wystąpiła w przypadku liczebności grzybów. Najwięcej antagonistycznych bakterii (Bacillus sp., Pseudomonas sp.) oraz grzybów (Gliocladium sp., Trichoderma sp.) w ryzosferze fasoli uzyskano, zaprawiając nasiona Biochikolem 020 PC.

Słowa kluczowe

EN

Phaseolus coccineus L.; chitosan; Zaprawa Oxafun T; healthiness; rhizosphere microorganisms

PL

Phaseolus coccineus L; chitozan; Zaprawa Oxafun T; zdrowotność; mikroorganizmy ryzosferowe

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. S
• Rocznik: 2009
• Tom: Vol. 16, nr 2
• Strony: 163--174
• Opis fizyczny: Bibliogr. 38 poz., rys., tab

Twórcy

autor

Patkowska E.

• Department of Phytopathology, University of Life Science in Lublin, ul. Leszczyńskiego 7, 20-069 Lublin,

Bibliografia

• [1] Patkowska E., Pięta D. and Pastucha A.: Diseases threatening plants of runner bean (Phaseolus coccineus L) cultivated in South-East Poland. Agronomijas vestis. Latvian J. Agronom., 2004, 7, 140-144.
• [2] Patkowska E., Pięta D. and Pastucha A: Biological control of runner bean (Phaseolus coccineus L) against soil-borne pathogenic fungi. Progress on Chemistry and Application of Chitin and its Derivatives. Monograph, M.M. Jaworska (ed.). Polish Chitin Society, Łódź, 2007, XII, 185-195.
• [3] Barabasz W.: Microorganisms as indicators of soil healthiness. Mat. of the Second National Conference on "Biological methods of estimating the state of the natural environment", Paradyż. AR Szczecin, 2004, pp. 84 (in Polish).
• [4] Martyniuk S., Masiak D., Stachyra A. and Myśków W.: Populations of the root zone microorganisms of various grasses and their antagonism towards Gaeumannomyces graminis var. tritici. Pr. IUNG, Pamięt.Puław., 1991,98, 139-144(in Polish).
• [5] Patkowska E.: The effect of biopreparations on the formation of rhizosphere microorganism populations of soybean (Glycine max (L) Merrill). Acta Sci. Pol., Hortorum Cultus, 2005, 4(2), 89-99.
• [6] Badura L.: Do we know all conditions of the microorganisms' functions in land eco-systems. Probl. Biol. Sci., 2004, 53( %), 373-379 (in Polish).
• [7] Diaz De Villegas M.E., Villa P. and Frias A: Evaluation of the siderophores production by Pseudomonas aeruginosa PSS. Revista Latinoamericana de Microbiologia, 2002, (44%), 112-117.
• [8] Lilieroth E., Baath E., Mariasson I. and Ludborg T.: Root exudation and rhizosphere bacterial abudance of barley (Hordeum vulgare L) in relation to nitrogen fertilization and root growth. Plant Soil, 1990, 127, 81-89.
• [9] Lewosz J.: Using antagonistic microorganisms towards plant pathogens in plant protection. Mat. from XLII Session of the Scientific Session of IOR. Poznań 2002, pp. 36 (in Polish).
• [10] Hou Xing Wei, Boyetchko S.M., Brick M., Olson D., Ross A and Hegedus D.: Characterization of the anti-fungal activity of a Bacillus spp. associated with sclerotia from Sclerotinia sclerotiorum. Appl. Microbiol. Biotech., 2006, 72(4), 644-653.
• [11] Srivastava R. and Shalini R.: Antifungal activity of Pseudomonas fluorescens against different plant pathogenic fungi. EJEFChe, 2008, 7(4), 2789-2796.
• [12] Pięta D. and Kęsik T.: The effect of conservation tillage on microorganism communities in the soil under onion cultivation. EJPAU, Hortieulture, 2007, 10(1), http://www.ejpau.media.pl/volume10/issue 1/art.-21.html
• [13] Me Quilken M.P., Gemmell J. and Lahdenperä M.L: Glioeladium catenulatum as a potential biological control agent of damping-off in bedding plants. J. Phytopathol., 2001, 149, 171-178.
• [14] Eziashi E.L., Urna N.U., Adekunle AA. and Airede C.E.: Effect of metabolites produced by Trichoderma species against Ceratocystis paradoxa in culture medium. Afric. J. Biotech., 2006, 5(9), 703-706.
• [15] Fiddman P.J., O'Neill T.M. and Rossall S.: Sereaning of bacteria for the suppression of Botrytis cinerea and Rhizoctonia solani on lettuce (Lectuca sativa) using leaf disc bioassays. Ann. Appl. Biol., 2000, 137(3), 223-235.
• [16] Goel A.K., Sindhu S.S. and Dadarwal K.R: Pigment diverse mutant of Pseudomonas sp. inhibition of fungal growth and stimulation of growth of Cicer arietinum. Biol. Plant., 2000, 43(4), 563-569.
• [17] Manwar A.V., Vaiganker P.D., Bhonge L.S. and Chincholkar S.B.: In vitro suppression of plant pathogens by siderophores of fluorescent Pseudomonas. Indian J. Microbiol., 2000, 40(2), 109-112.
• [18] Yeole R.D. and Dube H.C.: Siderophore-mediated antibiosis of rhizobacterial fluorescent pseudomonads against certain soil-borne fungal plant pathogens. J. Mycol. & Plant Pathol., 2000, 30(3), 335-338.
• [19] Chitarra G.S., Breeuwer P., Nout M.J.R., Van Aelst A.C., Ranbouts F.M. and Abee T.: An antifungal compound produced by Bacillus subtilis YM 10-20 inhibits germination of Penicillium roquefortii conidiospores. J. Appl. Microbiol., 2003, 94, 159-166.
• [20] Misko A.L. and Germida U.: Taxonomic and functional diversity of Pseudomonas isolated from roots of field-grown canola. FEMS Microbiol. Ecol., 2002, 42, 399-407.
• [21] Velazhahan R., Samiyappan R. and Vidhyasekaran P.: Relationship between antagonistic activities of Pseudomonas fluorescens isolates against Rhizoctonia solani and their production of lytic enzyme. J. Plant Dis. Protect., 1999, 106, 244-250.
• [22] Borkowski J., Felczyńska A. and Stepowski J.: Effect of different compounds Biochikol 020 PC, calcium nitrate, Tytanit and Pomonit on the healthiness and the yield of chinese cabbage. Progress on Chemistry and Application of Chitin and its Derivatives. Monograph, M.M. Jaworska (ed.). Polish Chitin Society, Łódź 2006, XI, 201-207.
• [23] Le Floch G., Rey P., Benizri E., Benhamou N., Tirilly Y. and Floch G.: Impact of auxin-compounds produced by the antagonistic fungus Pythium oligandrum or the minor pathogen Pythium group F on plant growth. Plant Soil, 2003, 257(2), 459-470.
• [24] Orlikowski L.B.: Effect of grapefruit extract on development of Phytophthora cryptogea and control of foot rot of gerbera. J. Plant Protect. Res., 2001, 41, 288-294.
• [25] Orlikowski L.B. and Jaworska-Marosz A.: Infulence of Pythium oligandrum on population of Fusarium oxysporum f. sp. dianthi and development of Fusarium wilt of carnation. Plant Protect. Sci., 2002, 38 (Special Issue 1), 209-211.
• [26] Orlikowski L.B. and Skrzypczak C.: Biocides in the control of soil-borne and leaf pathogens. Hortic. Veget. Grow., 2003, 22, 426-433.
• [27] Patkowska E.: The use of bioreparations in the control of soybean endangered by pathogenic soil-borne fungi. EJPAU, Hort., 2006, 9(1), http://www.ejpau.media.pl/volume9/issue 1/art.-19.html
• [28] Kurzawińska H. and Mazur S.: The effect of chitosan and Pythium oligandrum used in protection of potato tubers against late blith and soft rot. Progress on Chemistry and Application of Chitin and Its Derivatives. Monograp, M.M. Jaworska (ed.). Polish Chitin Society, Łódź 2008, XIII, 117-123.
• [29] Patkowska E.: The application of chitosan, Pythium oligandrum and grapefruit extract in the protection of common bean (Phaseolus vulgaris L.) from soil-borne phytopathogens. Progress on Chemistry and Application of Chitin and Its Derivatives. Monograph, M.M. Jaworska (ed.). Polish Chitin Society, Łódź 2008, XIII, 133-140.
• [30] Pięta D.: Healthiness and yielding of different cultivars of bean (Phaseolus vulgaris L.) depending on the rotation. Biul. Inst. Hodow. Aklimat. Rośl., 1992, 181/182, 261-267 (in Polish).
• [31] Sadowski Cz. and Rzekanowski S.: The effect of irrigation on the yielding and healthiness of rye on very light soil. Zesz. Probl. Post. Nauk Roln., 1989, 133, 363-370 (in Polish).
• [32] Pięta D., Pastucha A. and Patkowska E.: The efficiency of microbiological dressing of soybean seed (Glycine max (L.) Merrill) against root and stem base disease. Ann. Agric. Sci., Plant Protect., 1998, 95(112), 103-109.
• [33] Martin J.P.: Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Sci., 1950, 38, 215-220.
• [34] Mańka K. and Mańka M.: A new method for evaluating interaction between soil inhibiting fungi and plantpathogen. Bull.OILB/SROP, 1992, XV, 73-77.
• [35] Oktaba W.: Methods of mathematical statistics in experimentation. PWN, Warszawa 1987, 450 pp. (in Polish).
• [36] Patkowska E.: The effect of Oxafun T on the healthiness of pea (Pisum sativum L.) and on the formation of communities of rhizosphere microorganisms of this plant. Ecol. Chem. Eng., 2005, 12(4), 441-450.
• [37] Horoszkiewicz J. and Filoda G.: The effect of seed dressing on the healthiness of yellow lupine. Prog. Plant Protect/Post. Ochr. Roślin, 2001, 41(2), 718-721 (in Polish).
• [38] Pospieszny H.: Certain aspects of using chitosan in plant protection. Prog. Plant Protect., 1997, 37(1), 306-310 (in Polish).