Numerical simulation of multiple species detection using hydrodynamic/electrokinetic focusing

• Autorzy: Hahm J. , Beskok A.
• Języki publikacji: EN

Abstrakty

EN

In this paper we present the numerical simulation-based design of a new microfluidic device concept for electrophoretic mobility and (relative) concentration measurements of dilute mixtures. The device enables stationary focusing points for each species, where the locally applied pressure driven flow (PDF) counter balances the species' electrokinetic velocity. The axial location of the focusing point, along with the PDF flowrate and applied electric field reveals the electrokinetic mobility of each species. Simultaneous measurement of the electroosmotic mobility of an electrically neutral specie can be utilized to calculate the electrophoretic mobility of charged species. The proposed device utilizes constant sample feeding, and results in time-steady measurements. Hence, the results are independent of the initial sample distribution and flow dynamics. In addition, the results are insensitive to the species diffusion for large Peclet number flows (Pe > 400), enabling relative concentration measurement of each specie in the dilute mixture.

Słowa kluczowe

EN

species detection; electrophoresis; hydrodynamic/electrokinetic focusing; microfluidics

• Wydawca: Polska Akademia Nauk, Wydział IV Nauk Technicznych
• Czasopismo: Bulletin of the Polish Academy of Sciences. Technical Sciences
• Rocznik: 2005
• Tom: Vol. 53, nr 4
• Strony: 325--334
• Opis fizyczny: Bibliogr. 27 poz., 8 rys.

Twórcy

autor

Hahm J.

autor

Beskok A.

• Mechanical Engineering Department, Texas A&M University, Bio-Micro-Fluidics Laboratory, College Station TX 77843-3123, USA,

Bibliografia

• [1] R.F. Probstein, Physicochemical Hydrodynamics, Wiley and Sons Inc.,1997.
• [2] M. Melvin, Electrophoresis, John Wiley and Sons Inc, London, 1987.
• [3] W.L. Tseng and H.T. Chang, “A new stategy for optimizing sensitivity, speed, and resolution in capillary electrophoretic separation of DNA”, Electrophoresis 22, 763–770 (2001).
• [4] K. Kleparnik, Z. Mala, and P. Bocek, “Fast separation of DNA sequencing fragments in highly alkaline solutions of linear polyacrylamide using electrophoresis in bare silica capillaries”, Electrophoresis 22, 783–788 (2001).
• [5] M. Saur, B. Angerer, W. Ankenbauer, Z. Foldes-Papp, F. Gobel, K.T. Han, R. Rigler, A. Schulz, J. Wolfrum, and C. Zander, “Single molecule DNA sequencing in submicrometer channels: state of the art and future prospects”, Journal of Biotechnology 86(3), 181–201 (2001).
• [6] P.G. Righetti, Isoelectric Focussing: Theory, Methodology and Applications, Elsevier Biomedical, Amsterdam, 1983.
• [7] K. Macounova, C.R. Cabrera, M.R. Holl, and P. Yager, “Generation of natural pH gradients in microfluidic channels for use in soelectric focusing”, Anal. Chem. 72 (16), 3745–3751 (2000).
• [8] K. Macounova, C.R. Cabrera, and P. Yager, “Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF”, Anal. Chem. 73 (7), 1627–1633 (2001).
• [9] C.R. Cabrera, B. Finlayson, and P. Yager, “Formation of natural pH gradients in a microfluidic device under flow conditions: model and experimental validation”, Anal. Chem. 73 (3), 658–666 (2001).
• [10] C.H. Horiuchi, P. Dutta and C.F. Ivory, “Isoelectric focusing in a poly(dimethylsiloxane) microfluidic chip”, Analytical Chemistry 77 (5), 1303–1309 (2005).
• [11] R. Westermeier, Electrophoresis in Practice, VCH Publishers Inc, New York, 1990.
• [12] P. Janos, “Role of chemical equilibria in the capillary electrophoresis of inorganic substances”, Journal of Chromatography A 834, 3–20 (1999).
• [13] G.E. Karniadakis, A. Beskok, and N. Aluru, Microflows and Nanoflows: Fundamentals and Simulation, Springer-Verlag, New York, 2005.
• [14] P. Dutta and A. Beskok, “Analytical solution of time periodic electroosmotic flows: analogies to Stokes’ second problem”, Anal. Chem. 73, 5097–5102 (2001).
• [15] X. Niu, and Y.K. Lee, “Efficient spatial-temporal chaotic mixing in microchannels”, J. Micromech. Microeng. 13, 454–462 (2003).
• [16] A. Beskok and T.C.Warburton, “Unstructured hp finite-element scheme for fluid flow and heat transfer in moving domains”, Journal of Computational Physics 174, 492–509, (2001).
• [17] G.E. Karniadakis and S.J. Sherwin, Spectral/HP Element Methods for CFD, Oxford University Press, Oxford, 1999.
• [18] G.E. Karniadakis, M. Israeli, and S.A. Orszag, “High-order splitting methods for the incompressible Navier-Stokes equations”, Journal of Computational Physics 97, 414–443 (1991).
• [19] P. Dutta, A. Beskok, and T. Warburton, “Electroosmotic flow control in complex microgeometries”, J. Microelectromechanical Systems 11 (1), 36–44 (2002).
• [20] M.J. Kim, A. Beskok, and K.D. Kihm, “Electro-osmosis-driven micro-channel flows: a comparative study of microscopic particle image velocimetry measurements and numerical simulations”, Experiments in Fluids 33, 170–180 (2002).
• [21] E.V. Dose and G. Guiochon, “Time scales of transient processes in capillary electrophoresis”, Journal of Chromatography 652, 263–275 (1993).
• [22] D. Kaniansky, M. Masar, and J. Bielcikova, “Electroosmotic flow separation for capillary zone electrophoresis in a hydrodynamically closed separation system”, J. Chromatogr. A 792, 483–494 (1997).
• [23] Y. Liu, J.C. Fanguy, J.M. Bledsoe, and C.S. Henry, “Dynamic coating using polyelectrolyte multilayers for chemical control of electroosmotic flow in capillary electrophoresis microchips”, Analytical Chemistry 72 (24), 5939–5944 (2000).
• [24] J. Horvath and V. Dolnik, “Polymer wall coatings for capillary electrophoresis”, Electrophoresis 22, 644–655 (2001).
• [25] P. Righetti, C. Gelfi, B. Verzola, and L. Castelletti, “The state of art of dynamic coatings”, Electrophoresis 22, 603–611 (2001).
• [26] M.A. Holden, S. Kumar, E. Castellana, A. Beskok, and P.S. Cremer, “Generating fixed concentration arrays in a microfluidic device”, Sensors and Actuators B 92, 1999–2007 (2003).
• [27] W. Trimmer, Micromechanics and MEMS, Classic and Seminal Papers to 1990, IEEE Press, New York, 1997.