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Optimization of enzyme immobilization on interpolymer membranes

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The paper presents some preliminary results of assessing the efficiency of papain immobilization on amine modified polyethylene/poly(styrene-co-divinylbenzene) and polyethylene/poly- (vinylbenzyl chloride-co-divinylbenzene) membranes. The enzyme was immobilized due to adsorption. Three process variables were analyzed: concentration of papain in solution, time and temperature of sorption. In order to find the optimal values of these variables, statistical procedure was applied. It was shown that temperature insignificantly affected immobilization and its role could be accepted. The values of concentration and the time that allowed obtaining the most active preparations were also established.
Słowa kluczowe
Rocznik
Strony
221--228
Opis fizyczny
Bibliogr. 13 poz., tab., rys.
Twórcy
autor
  • Wrocław University of Technology, Faculty of Chemistry, 50-370 Wrocław, Poland
  • Wrocław University of Technology, Faculty of Chemistry, 50-370 Wrocław, Poland
autor
  • Wrocław University of Technology, Faculty of Chemistry, 50-370 Wrocław, Poland
autor
  • Wrocław University of Technology, Faculty of Chemistry, 50-370 Wrocław, Poland
  • .
Bibliografia
  • [1] HUANG P., BUTTERFIELD A., Optimization of covalently coupling enzymes to polymeric membranes: EPR studies of papain, J. Appl. Polym. Sci., 1993, 47, 1329–1338. Z K R L R P P P W 1990, – L B A G G
  • [2] EUSGEN M., GLODEK J., MILKA P., KREST I., Immobilization of enzymes on PTFE surfaces, Biotechnol. Bioeng., 2001, 72, 530–535.
  • [3] UCKA M., POŹNIAK G., TURKIEWICZ B., TROCHIMCZUK W., Ultrafiltration membranes from polysulfone/aminated polysulfone blends with proteolytic activity, Enzyme Microb. Technol., 1996, 18, 477–481.
  • [4] I Y.F., JIA Y., LI J.R., LI Y.Z., Papain immobilization on a nitrilon fiber carrier containing primary amine groups, Biotechnol. Appl. Biochem., 2001, 33, 29–34.
  • [5] UCKA M., LAMER-ZARAWSKA E., MALISZEWSKA I., TURKIEWICZ B., Optimization of growth and hydrolytic enzymes production by Fusarium culmorum using response surface method, Bioprocess Eng., 1998, 19, 229–232.
  • [6] OŹNIAK G., TROCHIMCZUK W., Interpolymer anion exchange membranes. I. Properties of weak base membranes on polyethylene modified by styrene and divinylbenzene in the presence of diluents, Angew. Macromol. Chem., 1980, 92, 155–168.
  • [7] OŹNIAK G., TROCHIMCZUK W., Chloromethylation of polyethylene/poly(styrene-co-divinylbenzene) system, J. Appl. Polym. Sci., 1982, 27, 1833–1838.
  • [8] OŹNIAK G., TROCHIMCZUK W., Tubular interpolymer ion-exchange membranes. Donnan dialysis through strong-base membranes, J. Membr. Sci., 1990, 49, 55–68.
  • [9] ARSHAWSKY A., KAHANA N., DESHE A., GOTTLIEB E., ARAD-YELLIN R., Halomethylated polysulfone reactive intermediates to neutral and ionic film-forming polymers, J. Polym. Sci., Polym. Ed., 28, 2885 2905.
  • [10] OWRY O.H., ROSENBROUGH N.J., FARR A.L., RANDALL R.J., Protein measurement with folin phenol reagent, J. Biol. Chem., 1951, 193, 265–275.
  • [11] ROWN M.E., The colorimetric determination of arginine ester hydrolysis by human sera, J. Lab. Clin. Med., 1960, 35, 616–619.
  • [12] RASARATNAM V., ALAEV I.Yu., MATTIASON B., Reversibly soluble biocatalyst: optimization of trypsin coupling to Eudargit S-100 and biocatalyst activity in soluble and precipitated form, Enzyme Microb. Technol., 2001, 27, 254–263.
  • [13] ANAPATHI-DESAI S., BUTTERFIELD A., BHATTACHARYYA D., Kinetics and active fraction determination of protease enzyme immobilized on functionalized membranes: mathematical modeling and experimental results, Biotechnol. Prog., 1998, 14, 865–873.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BPG5-0011-0044
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