Effects of supersonic treatment on lactoferrin separation from whey waste using reversed micellar system

• Autorzy: Inoue H. , Yamasaki M. , Lakshmi D. S. , Kojo T. , Yamagishi S. I.
• Języki publikacji: EN

Abstrakty

EN

A cationic oleic acid reversed micellar system was used to simplify and enhance the purification of bovine lactoferrin (Lfn) from whey. The rate of Lfn extraction by the application of 30 kHz supersonic treatment to the organic solvent phase (P < 0.01) was by 1.5 time higher compared to non-supersonic treatment. An increase in water content and the light scatter were observed in the organic phase when the supersonic treatment was applied. An increase in the efficiency of Lfn extraction by supersonic treatment of an organic phase was attributable to increased water dispersion in the organic solvent phase and an increase in the orientation of the cationic oleic acid towards microparticles of water in organic solvent phase.

Słowa kluczowe

EN

lactoferrin; whey; reversed micellar system; supersonication; lipid

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Environment Protection Engineering
• Rocznik: 2005
• Tom: Vol. 31, nr 3-4
• Strony: 5--12
• Opis fizyczny: Bibliogr. 12 poz., tab., rys.

Twórcy

autor

Inoue H.

• Radioisotope Institutes for Basic and Clinical Medicine, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Japan. tel: [phone]; FAX: [phone]

autor

Yamasaki M.

• Radioisotope Institutes for Basic and Clinical Medicine, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Japan. tel: [phone]; FAX: [phone]

autor

Lakshmi D. S.

• Radioisotope Institutes for Basic and Clinical Medicine, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Japan. tel: [phone]; FAX: [phone]

autor

Kojo T.

• Radioisotope Institutes for Basic and Clinical Medicine, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Japan. tel: [phone]; FAX: [phone]

autor

Yamagishi S. I.

• Radioisotope Institutes for Basic and Clinical Medicine, Department of Medicine, Kurume University School of Medicine, 67 Asahi-machi, Kurume, 830-0011, Japan. tel: [phone]; FAX: [phone]

Bibliografia

• [1] LEVAY P.F., VILJOEN M., Lactoferrin: a general review, Haematologica, 1995, 80, pp. 252–267.
• [2] BROOK J.H., The physiology of lactoferrin, Biochemical. Cell. Biology, 2002, 80, pp. 1–6.
• [3] BIRGENS H.S., Lactoferrin in plasma measured by an ELISA technique: evidence that plasma lactoferrin is an indicator of neutrophil turnover, Scandinavian Journal of Haematology, 1985, 34, pp. 326–331.
• [4] INOUE H., SAKAI M., KAIDA Y., KAIBARA K., Blood lactoferrin induced by running exercise in normal volunteers: anitibacterial activity, Clin. Chim. Acta, 2004, 341, pp. 165–172.
• [5] INOUE H., YAMASAKI M., LAKSHMI D.S., Lactoferrin Extraction using Oleic Acid Sodium Salt and Tetraphenylboron Sodium Reverse Micellar System, submitted.
• [6] YAMADA Y., KUBOI R., KOMASAWA I., Extraction of enzymes and their activities in AOT reverse micellar systems modified with nonionic surfactants, Journal of Chemical Engineering of Japan, 1994, 27(3), pp. 404–409.
• [7] GOTO M., ONO T., NAKASHIO F., HATTON T.A., Design of surfactants suitable for protein extraction by reversed micelles, Biotechnology and Bioengineering, 1997, 54, pp. 26–32.
• [8] HATTON T.A., Surfactant-Based Separation Processes, Scamehorn J.F., Harwell J.H. (editors), Marcel Dekker, New York, 1989, pp. 55–90.
• [9] AWAD S.B., An ultrasonic semi-aqueous alternative to vapor degreasing, Precision Cleaning, 1993, 1(1), p. 75.
• [10] PRAUSNITZ J.M., LICHTENTHALER R.N., DE AZEVEDO E.G., Molecular Thermodynamics of Fluid Phase Equilibria, 2nd ed., Prentice-Hall, 1986.
• [11] ASHRAFIZADEH S.N., KHOSHKBARCHI M.K., Modeling and experimental data for the reverse micellar extraction of protein using a new surfactant, Separation Science and Technology, 1998, 33(16), pp. 2579–2595.
• [12] RONG L., YAMANE T., TAKEUCHI H., A new reversed micellar system from di(2-ethylhexyl)phospho- -rothioic acid: its application to extraction of hemoglobin, Journal of Chemical Engineering of Japan, 1998, 31(3), pp. 434–439