Mutagenicity of pollutants and their fractions adsorbed on airborne par-ticulate in the centre of Wrocław (Poland)

• Autorzy: Jadczyk P. , Kucharczyk J.

Warianty tytułu

PL

Mutagenność zanieczyszczeń zaadsorbowanych na pyle zawieszonym w centrum Wrocławia (Polska) i ich frakcji

• Języki publikacji: EN

Abstrakty

EN

Mutagenicity of pollutants and their fractions adsorbed on suspended particulate matter < 10 žm (PM10) in the centre of Wrocław was studied using the Ames test (SW Poland, 642 000 inhabitants). Particulate matter was sampled by the method of high-volume aspirometry in summer and in winter. Samples were extracted with dichloromethane. Extract was separated into fractions with sequential elution by solvent chromatography. In the Ames test there was used Salmonella typhimurium TA 98 strain and YG 1021 and YG 1024 strains of increased sensitivity to mutagenic activity of nitro PAHs. In order to perform activation of promutagenes, S9 fraction obtained from rat liver was used. Pollutants adsorbed on particulate matter displayed greater mutagenicity in winter than in summer. In summer, mutagenic activity was displayed exclusively by polar pollutants, in winter by PAHs and polar compounds. Among them there were direct and indirect mutagenes. Significant role in mutagenicity of pollutants adsorbed on particulate matter played nitro PAHs. Mutagenic effect found due to the Ames test was the result of total activity of many mutagenes displaying synergistic and antagonistic actions in the mixture.

PL

Korzystając z testu Amesa, zbadano mutagenność frakcji chromatograficznych zanieczyszczeń zaadsorbowanych na pyle zawieszonym PM10 w centrum Wrocławia (642 000 mieszkańców). Pył pobierano metodą wysokowydajnej aspirometrii latem i zimą. Próby ekstrahowano dichlorometanem. Rozdziału na frakcje dokonano metodą chromatografii kolumnowej. W teście Amesa zastosowano szczep Salmonella typhimurium TA 98 oraz szczepy YG 1021 i YG 1024 o podwyższonej wrażliwości na mutagenne działa-nie nitropochodnych WWA. Aby aktywować promutageny, stosowano frakcję S9 uzyskaną z wątroby szczurów. Zanieczyszczenia zaadsorbowane na pyle wykazywały większą mutagenność zimą niż latem. Latem aktywność mutagenną wykazywały wyłącznie zanieczyszczenia polarne, zimą WWA i związki polarne. Były wśród nich mutageny bezpośrednie i pośrednie. Znaczący udział w mutagenności zanieczyszczeń zaadsorbowanych na pyle miały nitropochodne WWA. Efekt mutagenny stwierdzany za pomocą testu Amesa był wynikiem łącznego działania wielu mutagenów wykazujących w mieszaninie działania synergistyczne i antagonistyczne.

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Environment Protection Engineering
• Rocznik: 2005
• Tom: Vol. 31, nr 2
• Strony: 77--92
• Opis fizyczny: Bibliogr. 25 poz., tab., rys.

Twórcy

autor

Jadczyk P.

• Institute of Environment Protection Engineering, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland

autor

Kucharczyk J.

• Custom Laboratory, Custom-House in Wrocław, Skarżyńskiego 36, 54-530 Wrocław, Poland

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