Methods of examination of genotoxicity of atmospheric pollutants

• Autorzy: Jadczyk P.

Warianty tytułu

PL

Metody badania genotoksyczności zanieczyszczeń atmosfery

• Języki publikacji: EN

Abstrakty

EN

Current physicochemical monitoring of atmospheric pollutants fully reflects their biological activity. This should be supplemented with evaluation of mutagenicity and carcinogenicity of those pollutants by means of biotests. Test selection should take into consideration the sensitivity of test organisms to genotoxic activity of atmospheric pollutants, conformity of the results with those of other biotests, common use of environmental mutagenesis and carcinogenesis in the tests with special consideration of atmospheric pollutants, possibility of examining the activity of atmospheric pollutants independent of atmospheric conditions, short duration of the test cycle, relatively low cost, reducing the suffering of laboratory animals to minimum. Those criteria are best fulfilled by the following tests on microorganisms and tissue cultures: the Ames test with Salmonella typhimurium, sister chromatide exchange induction and canceration in cells of human respi-ratory systems and identification of DNA adducts in human organisms.

PL

Stosowany obecnie monitoring fizykochemicznych zanieczyszczeń atmosfery nie odzwierciedla w pełni ich aktywności biologicznej. Powinien on być uzupełniony oceną mutagenności i kancerogenno-ści tych zanieczyszczeń za pomocą biotestów. Wybierając biotesty, należy wziąć pod uwagę: wrażliwość testowanych organizmów na genotoksyczne działanie atmosfery, zgodność otrzymanych wyników z wynikami innych biotestów, powszechność stosowania testów w badaniu mutagenezy i kancerogenezy środowiskowej ze szczególnym uwzględnieniem zanieczyszczeń atmosfery, możliwość badania aktywności zanieczyszczeń atmosfery niezależnie od warunków atmosferycznych, krótkotrwałość cyklu badawczego, relatywnie niski koszt oraz ograniczenie do niezbędnego minimum cierpienia zwierząt laboratoryjnych. Kryteria te w największym stopniu spełniają testy na mikroorganizmach i kulturach tkankowych: test Amesa na Salmonella typhimurium, indukowanie wymiany chromatyd siostrzanych i transformacji nowotworowej w komórkach narządów oddechowych człowieka oraz identyfikacja adduktów DNA u ludzi.

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Environment Protection Engineering
• Rocznik: 2003
• Tom: Vol. 29, nr 3-4
• Strony: 97--112
• Opis fizyczny: Bibliogr. 94 poz.

Twórcy

autor

Jadczyk P.

• Institute of Environment Protection Engineering, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.

Bibliografia

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