Specific for DNA damages GFP microbial biosensor as a tool for genotoxic action assessment of environmental pollution

• Autorzy: Matejczyk M.
• Języki publikacji: EN

Abstrakty

EN

In the presented paper, autofluorescent reporter of Escherichia coli K-12 recA::gfpmut2 strain, which contained a plasmid-borne transcriptional fusion between DNA-damage inducible recA promoter involved in the SOS regulon response and fast folding GFP variant reporter gene-gfpmut2, have been used. GFP-based bacterial biosensors allowed the detection of bacterial cells response to selected tested genotoxic compounds such as mitomycin C (MMC), actinomycin D, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and formaldehyde (CH2O). Experiment indicated that E. coli K-12 recA::gfpmut2 biosensor strain is more specific and sensitive for especially two genotoxins: actinomycin D and MNNG and with very low response to other agents. So it was concluded that for formaldehyde and MMC E. coli K-12 recA::gfpmut2 genetic system is disqualified for genotoxicity screening.

Słowa kluczowe

EN

DNA damage; genotoxicity; recA promoter; SOS response

PL

uszkodzenia DNA; genotoksyczność

• Wydawca: Oficyna Wydawnicza Politechniki Białostockiej
• Czasopismo: Budownictwo i Inżynieria Środowiska
• Rocznik: 2010
• Tom: Vol. 1, no. 4
• Strony: 319--326
• Opis fizyczny: Bibliogr. 41 poz., rys.

Twórcy

autor

Matejczyk M.

• Bialystok Technical University, Faculty of Civil Engineering and Environmental Engineering,

Bibliografia

• 1. Ahn J-M., E. T. Hwang, CH-H Youn, D. L. Banu, B. CH. Kim, J. H. Niazi and M. B. Gu. (2009). Prediction and classification of the modes of genotoxic action using bacterial biosensors specific for DNA damages. Biosens. Bioelectron. 25: 767-772.
• 2. Bae J.H., M. Rubini, G. Jung, G.Wiegand, M. H. J. Seifert, M. K. Azim, J.-S. Kim, A. Zumbusch, T. A. Holak, L. Moroder, R. Huber and N. Budisa. (2003). Expansion of the genetic code enables design of a novel “gold” class of green fluorescent proteins. J. Mol. Biol. 328: 1071-1081.
• 3. Baumstark-Khan Ch., E. Rabbow, P. Rettberg and G. Horneck. (2007). The combined bacterial Lux-Fluoro test for the detection and quantification of genotoxic and cytotoxic agents in surface water: Results from the “Technical Workshop on Genotoxicity Biosensing”. Aqua. Toxicol. 85: 209-218.
• 4. Belkin S. (2003). Microbial whole-cell sensing systems of environmental pollutants. Cur. Opin. Microbiol. 6: 206-212.
• 5. Bongaerts R. J. M., I. Hautefort, J. M. Sidebotham and J. C. D. Hinton. (2002). Green fluorescent protein as a marker for conditional gene expression in bacterial cells. Meth. Enzymol. 358: 43-47.
• 6. Brander W.T. (2001). Mitomycin C: a clinical update. Canc. Treat. Rev. 27: 35-50.
• 7. Casavanth C., D. Thompson, G. A. Beatie, G. J. Philips and L. J. Halverson. (2003). Use of site-specific recombination-based biosensor for detecting toluene and related compounds on roots. Environm. Microbiol. 4: 238-249.
• 8. Cha H. J., R. Srivastava, V. Vakharia, G. Rao and W. Bentley. (1998). Green fluorescent protein as a noninvasive stres probe in resting Escherichia coli cells. Appl. Environm. Microbiol. 65: 409-414.
• 9. Chirico G., F. Cannone, S. Beretta, A. Diaspro, B. Campanini, S. Bettati, R. Ruotolo and A. Mozzareli. (2002). Dynamice of green fluorescent protein mutant2 in solution, on spincoated glasses, and encapsulated in wet silica gels. Protein Scien. 11: 1152-1161.
• 10. Contag CH. H., D. Jenkins, P. R. Contag and R. S. Negrin. (2000). Use of reporter genes for optical measurements of neoplastic disease in vivo. Neoplasia. 2, 1-2: 41-52.
• 11. D'Souza S. F. (2001). Microbial biosensors. Biosens. Bioelectron. 16: 337-353.
• 12. Errampalli D., K. Leung, M. B. Cassidy, M. Kostrzyńska, M. Blears, H. Lee and J. T. Trevors. (1999). Applications of green fluorescent protein as a molecular marker in environmental microorganism. J. Microbiol. Meth. 35:187-199.
• 13. Girotti S., E. N. Feeri, M. G. Fumo and E. Maiolini. (2008). Monitoring of environmental pollutants by bioluminescent bacteria. Anal. Chim. Act. 608: 2-29.
• 14. Gu M. B., R. J. Mitchell and B. C. Kim. (2004). Whole-cellbased biosensors for environmental biomonitoring and application. Adv. Biochem. Engin. Biotechnol. 87: 269-305.
• 15. Hansen L.H. and S. J. Sørensen. (2001). The use of whole-cell biosensors to detect and quantify compounds or conditions affecting biological systems. Microb. Ecol. 42: 483–494.
• 16. Hazen T.C. and D. A. Stahl. (2006). Using the stress response to monitor process control: pathways to more effective bioremediation. Cur. Opin. Biotechnol. 17: 285–290.
• 17. Jansson J. K. (2003). Marker and reporter genes: illuminating tools for environmental microbiologists. Cur. Opin. Microbiol. 6: 310-316.
• 18. Kain S.R. (1999). Green fluorescent protein (GFP): applications in cell-based assays for drug discovery. Drug Discov. Tod. 4(7): 304-312.
• 19. Kim B. C. and M. B. Gu. (2003). A bioluminescent sensor for high throughput toxicity classification. Biosens. Bioelectron. 160: 445-457.
• 20. Kostrzyńska M., K. T. Leung, H. Lee and J. T. Trevors. (2002). Green fluorescence protein based biosensor for detecting SOS-inducing activity of genotoxic compounds. J. Microbiol. Meth. 48: 43-51.
• 21. Kuang Y., I. Biran and D. R. Walt. (2004). Living bacterial cell array for genotoxins monitoring. Anal. Chem. 76: 2902-2909.
• 22. Lee J. H., R. J. Mitchell, B. CH. Kim, D. C. Cullen and M. B. Gu. (2005). A cell array biosensor for environmental toxicity analysis. Biosens. Bioelectron. 21: 500-507.
• 23. Lei Y., W. Chen, A. Mulchandani. (2006). Microbial biosensors. Anal. Chim. Act. 568: 200–210.
• 24. Mao Y. M., D. H. Varoglu and D. H. Sherman. (1999). Molecular characterization and analysis of the biosynthetic cluster for the antitumor antibiotic mitomycin C from Streptomyces lavendulae NRRL2564. Chem. Biol. 6: 251-263.
• 25. Matejczyk M. (2004). Bakteryjne biosensory. Post. Mikrobiol. 2(43):155-165.
• 26. Matejczyk M. and Rosochacki S. J. (2006). Application of plasmid-borne green fluorescent protein-based bacterial biosensors for benzene and its selected derivatives detection in water ecosystems. Pol. J. Environ. Stud. 15(5D), 703-707.
• 27. Matejczyk M. and S. J. Rosochacki. (2007). Gen gfp jako fluorescencyjne narzędzie w analizie ekspresji genów i konstrukcji biosensorów. Biotechnol. 1(76): 53-62.
• 28. Mitchell R. J. and M. B. Gu. (2003). Construction and characterisation of novel dual stress-responsive bacterial biosensors. Biosens. Bioelectron. 19: 977-985.
• 29. Neuss S. and G. Speit. (2008). Further characterization of the genotoxicity of formaldehyde in vitro by the sister chromatid exchange test and co-cultivation experiments. Mutagen. 23(5): 355-357.
• 30. Norman A., L. H. Hansen and S. J. Sørensen. (2006). A flow cytometry-optimized assay using an SOS-green fluorescent protein (SOS-GFP) whole-cell biosensor for the detection of genotoxins in complex environments. Mutat. Res. 603: 164-172.
• 31. Ptitsyn L.R., G. Horneck, O. Komova, S. Kozubek, E. A. Krasavin, M. Bonev and P. Rettberg. (1997). A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells. Appl. Environ. Microbiol. 63(11): 4377-4384.
• 32. Rogers K.R. (2006). Recent advances in biosensor techniques for environmental monitoring. Anal. Chim. Act. 568: 222-231.
• 33. Ron E. Z. (2007). Biosensing environmental pollution. Cur. Opin. Biotechnol. 18: 252-256.
• 34. Rosochacki S.J. and M. Matejczyk. (2002). Green fluorescent protein as a molecular marker in microbiology. Acta Microbiol. Pol. 51(3): 205-216.
• 35. Sørensen S.J., M. Burmølle and L. H. Hansen. (2006). Making bio-sense of toxicity: new developments in whole-cell biosensors. Cur. Opin. Biotechnol. 17: 11-16.
• 36. Stiner L. and L. J. Halverson. (2002). Development and characterisation of a green fluorescent protein-based bacterial biosensor for bioavailable toluene and related compounds. Appl. Environ. Microbiol. 68: 1962-1971.
• 37. Turan T., O. Karacay, G. Tulunay, N. Boran, S. Koc, S. Bozon and M. Kose. (2006). Results with EMA/CO (etoposide, methotrexate, actinomycin D, cyclophosphamide, vincristine) chemotherapy in gestational trophoblastic neoplasia. Int. J. Gynecol. Cancer. 16(3): 1432-1438.
• 38. Vollmer A. C. and T. Van Dyk. (2004). Stress responsie bacteria: biosensors as environmental monitors. Adv. Microbiol. Physiol. 49: 131-174.
• 39. Willardson B. M., J. F. Wilkins, T. A. Rand, J. M. Schupp, K. K. Hill, P. Keim and P. J. Jackson. (1998). Development and testing of a bacterial biosensor for toluene-based environmental contaminants. Appl. Environ. Microbiol. 64: 1006-1012.
• 40. Yagi K. (2007). Applications of whole-cell bacterial sensors in biotechnology and environmental science. Appl. Microbiol. Biotechnol. 73: 1251-1258.
• 41. Zaslaver A., A. E. Mayo, R. Rosemberg, P. Bashkin, H. Sberro, M. Tsalyuk, M. G. Surette and U. Alon. (2004). Just-in-time transcription program in metabolic pathways. Nat. Genet. 36(5): 486 – 491.