Phylogenetic diversity and abundance of bacteria from surface microlayer and subsurface water in eutrophic lake

• Autorzy: Walczak M. , Swiontek Brzezinska M.
• Języki publikacji: EN

Abstrakty

EN

The bacteria from different phylogenetic groups were studied in surface microlayer (SM, up to 100 [mu]m ) versus subsurface water (SW - 20 cm) in eutrophic lake from spring to autumn of 2007. Abundance of bacteria was determined using a combination of direct counting of 4', 6-diamidino-2-phenylindole and the phylogenetic diversity was determined in fluorescence in situ hybridization (FISH) method with group-specific, fluorescently labeled oligonucleotide probes. The numbers of DAPI bacteria varied between 4.75 and fluorescence in situ hybridization (FISH) demonstrated that Eubacteria constituted the majority of the whole bacterial population and their percentage share ranged from 59 to 75%. Abundances of alpha- beta-Proteobacteria and Cytophaga-Flavobacteria groups varied across seasons, layers, and lacustrine zones. The lowest number of alpha-Proteobacteria group bacteria was observed in spring (SM - 0.2 x 10[^6], SW - 0.16 x 10[^6] cells cm[^-3]), whereas the highest in autumn (SM - 0.62 x 10[^6], SW - 1.6 x 10[^6] cells cm[^-3]). The percentage share of these groups of bacteria in the Eubacteria domain was lower in spring (20.50%) than in summer and autumn (from 65 to over 80%). No fixed difference between the composition of SM and SW bacteria was noticed. Seasonally occurred changes are similar in both layers.

Słowa kluczowe

EN

bacterial communities; biodiversity of bacteria; FISH; surface microlayer

PL

bioróżnorodność bakterii; mikrowarstwa powierzchniowa; zbiorowiska bakterii

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2010
• Tom: Vol. 58, nr 1
• Strony: 177--186
• Opis fizyczny: Bibliogr. 30 poz.,Rys., tab.,

Twórcy

autor

Walczak M.

autor

Swiontek Brzezinska M.

• Department of Environmental Microbiology and Biotechnology Institute of Ecology and Environment Protection, Nicolaus Copernicus University Gagarina 9, 87-100 Toruń, Poland,

Bibliografia

• 1. Alfreider A., Pernthaler J., Amann R., Sattler B., Glöckner F.O., Wille A., Psenner R. 1996 – Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization – Appl. Environ. Microbiol. 62: 2138–2144.
• 2. Amann R.I., Krumholz L., Stahl D.A. 1990 – Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic and environmental studies in microbiology – J. Bacteriol. 172: 762–770.
• 3. Amann R.I., Ludwig W., Schleifer K.H. 1995 – Phylogenetic identification and in situ detection of individual microbial cells without cultivation – Microbiol. Rev. 59: 143–169.
• 4. Anton J., Llobet-Brossa E., Rodriguez-Valera F., Amann R. 1999 – Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds – Environ. Microbiol. 1: 517–523.
• 5. Araya R., Tani K., Takagi T., Yamaguchi N., Nasu M. 2003 – Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis – FEMS Microbiol. Ecol. 43: 111–119.
• 6. Brosius J., Dull T.L., Sleeter D.D., Noller H.F. 1981 – Gene organization and primary structure of a ribosomal operon from Escherichia coli – J. Mol. Biol. 148: 107–127.
• 7. Brümmer I.H.M., Fehr W., Wagner-Döbler I. 2000 – Biofilm community structure in polluted rivers: abundance of dominant phylogenetic groups over a complete annual cycle – Appl. Environ. Microb. 66: 3078–3082.
• 8. Cottrell M.T., Kirchman D.L. 2000 – Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization – Appl. Environ. Microb. 66: 5116–5122.
• 9. Falkowska L. 2001 – 12-hour cycle of matter transformation in the sea surface microlayer in the offshore waters of Gdańsk Basin (Baltic Sea) during spring – Oceanologia, 43: 201–222.
• 10. Garrett W.P. 1965 – Collection of slick-forming materials from the sea surface – Limnol. Oceanogr. 10: 602–605.
• 11. Glöckner F.O., Amann R., Alfreider A., Pernthaler J., Psenner R., Trebesius K., Schleifer K.H. 1996 – An in situ hybridization protocol for detection and identification of planktonic bacteria – Syst. Appl. Microbiol. 19: 403–406.
• 12. Glöckner F.O., Fuchs B.M., Amann R. 1999 – Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization – Appl. Environ. Microb. 65: 3721–3726.
• 13. Hillbricht-Ilkowska A., Kostrzewska-Szlakowska I. 2004 – Surface microlayer in lakes of different trophic status: nutrients concentration and accumulation – Pol. J. Ecol. 52: 461–478.
• 14. Höfle M.G., Haas H., Dominik K. 1999 – Seasonal dynamics of bacterioplankton community structure in a eutrophic lake as determined by 5S rRNA analysis – Appl. Environ. Microbiol. 65: 3164–3171.
• 15. Joint R., Morris R. 1982 – The role of bacteria in the turnover of organic matter in the sea – Oceanogr. Mar. Biol. Ann Rev 20: 65–118.
• 16. Kirchman D.L. 2002 – The ecology of Cytophaga–Flavobacteria in aquatic environments – FEMS Microbiol. Ecol. 39: 91–100.
• 17. Knoll S., Zwisler W., Simon M. 2001 – Bacterial colonization of early stages of limnetic diatom microaggregates – Aquatic Microbial Ecol. 25: 141–150.
• 18. Kostrzewska-Szlakowska I. 2003 – Mikrowarstwa powierzchniowa wód: charakterystyka i metody badań [A surface microlayer of waters: characteristics and research methods] – Wiad. Ekol. 49: 183–202 (in Polish, English summary).
• 19. Manz W., Wendt-Potthoff K., Neu T.R., Szewczyk U., L awrence J.R. 1999 – Phylogenetic composition, spatial structure and dynamics of lotic bacterial biofilms investigated by fluorescent in situ hybridization and confocal laser scanning microscopy – Microb. Ecol. 37: 225–237.
• 20. Olapade O.A., Leff L.G. 2004 – Seasonal dynamics of bacterial assemblages in epilithic biofilms in a north-eastern Ohio stream – J. North Am. Benthol. Soc. 23: 686–700.
• 21. Pearce D.A. 2003 – Bacterioplankton community structure in a maritime Antarctic oligotrophic lake during a period of holomixis, as determined by Denaturing Gradient Gel Electrophoresis (DGGE) and Fluorescence in Situ Hybridization (FISH) – Microbial. Ecol. 46: 92–105.
• 22. Pernthaler J., Glöckner F.O., Unterholzner S., Alfreider A., Psenner R., Amann R. 1998 – Seasonal community and population dynamics of pelagic Bacteria and Archaea in a high mountain lake – Appl. Environ. Microbiol. 64: 4299–4306.
• 23. Plasquellec A., Beucher H., Le Lay C., Cleret J. 1991 – Quantitative and qualitative bacteriology of the marine water surface microlayer in a sewage – polluted area – Mar. Environ. Res. 31: 227–239.
• 24. Porter K.G., Feig Y.S. 1980 – The use of DAPI for identifying and counting aquatic microflora – Limnol. Oceanogr. 25: 943–948.
• 25. Schweizer B., Huber I., Amann R., Ludwig W., Simon M. 2001 – a-and b-proteobacteria control the consumption and release of amino acids on lake snow aggregates – Appl. Environ. Microbiol. 67: 632–645.
• 26. Šimek K., Pernthaler J., Weinbauer M., Hornák K., Dolan J., Nedoma J., Masin M., Amann R. 2001 – Changes in bacterial community composition and dynamics and viral mortality rates associated with enhanced flagellate grazing in a mesoeutrophic reservoir – Appl. Environ. Microbiol. 67: 2723–2733.
• 27. Šimek K., Kojecká P., Nedoma J., Hartmann P., Vrba J. 1999 – Shifts in bacterial community composition associated with different microzooplankton size fractions in a eutrophic reservoir – Limnol. Oceanogr. 44: 1643–1644.
• 28. Walczak M., Donderski W. 2005 – Bacterioneuston of water bodies – Post. Mikrobiol. 3: 275–288.
• 29. Weiss P., Schweitzer B., Amann R., Simon M. 1996 – Identification in situ and dynamics of bacteria on limnetic organic aggregates (lake snow) – Appl. Environ. Microbiol. 62: 1998–2005.
• 30. Zwisler W., Selje N., Simon M. 2003 – Seasonal patterns of the bacterioplankton community composition in a large mesotrophic lake – Aquat. Microbial. Ecol. 31: 211–225.