impact of UV mediated hydrogen peroxide on culturable bacteria in the surface microlayer of eutrophic lake

• Autorzy: Walczak M. , Swiontek Brzezinska M.
• Języki publikacji: EN

Abstrakty

EN

Hydrogen peroxide (H2O2) formation in surface waters is initiated by the absorption of sunlight by dissolved organic matter (DOM). The fraction of the DOM pool that interacts with sunlight, referred to as chromophoric dissolved organic matter, impacts the optical properties of surface waters. Second source of H2O2 is wet and dry deposition of photogenerated substance in the atmosphere and biological production. The study examined the concentration of hydrogen peroxide in water from the surface microlayer (SM) (<100 [my]m) and subsurface water (SSW) (25 cm) in the typical eutrophic (TOC 5-15 mg dm[^-3]; chlorophyll 5-26 [my]g dm[^-3], water transparency 0.6-1.0 m) lake as well as the impact of this compound on occurrence and survivorship of catalase-positive and catalase-negative bacteria isolated and cultured on the TSA medium (Difco). The experimental H2O2 concentrations ranged between 500-5000 nM. The concentration of H2O2 in analyzed water samples clearly increased in day-time hours and was different in May, July and October. The highest natural concentration of H2O2 (700 nM) was observed in SM water in summer in afternoon hours. During that period, 100% of bacterial populations found in SM water produced catalase. The experiments confirmed that environmental concentrations of H2O2 caused no considerable decrease in survivorship of culturable bacteria, while concentrations exceeding 1000 nM were lethal for the majority of catalasenegative bacteria, but not for catalase-positive bacteria.

Słowa kluczowe

EN

bacteria; catalase; hydrogen peroxide; surface microlayer

PL

bakteria; katalaza; mikrowarstwa powierzchniowa; nadtlenek wodoru

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2009
• Tom: Vol. 57, nr 3
• Strony: 547--554
• Opis fizyczny: Bibliogr. 19 poz.,Tab., wykr.,

Twórcy

autor

Walczak M.

autor

Swiontek Brzezinska M.

• Department of Environmental Microbiology and Biotechnology Institute of Ecology and Environment Protection, Nicolaus Copernicus University Gagarina 9, 87-100 Toruń, Poland,

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