Culturable proteolytic bacteria and the activity of luceine aminopeptidase in river ecosystem

• Autorzy: Skórczewski P. , Mudryk Z.
• Języki publikacji: EN

Abstrakty

EN

The study determined the numbers ad distribution of culturable heterotrophic and proteolytic bacteria and the level of leucine aminopeptidase activity in the water of the Słupia River within the town of Słupsk (Northern Poland). River Słupia is 138.6 km long and flowing into the Baltic Sea. The average water discharge of the river is 15.5 m[^3]s[^-1] and its slope is about 1.3% which gives it a mountainous character. The numbers of culturable heterotrophic bacteria was determined on iron-peptone agar (IPA) medium and the numbers of culturable proteolytic bacteria was assayed in IPA medium enriched with gelatin. Potential leucine aminopeptidase activity was carried out with the use of fluorescently labelled model substrate MCA-leucine. The numbers of heterotrophic bacteria ranged between 033 to 183.0 x 10[^3] cells cm [^-3] (maximum in spring, minimum in winter); the abundance of culturable protteolytic bacteria ranged 0.50 to 31.8 x 10[^3] cells cm[^-3] and the maximum was noted in spring, the lowest in summer. The activity of extracellular leucine aminopeptidase ranged from 2.34 to 6.87 [my]M MCA dm[^-3] h[^-1] and the highest value was noted in spring while the lowest were noted in winter and summer. The values of bacteriological parameters and of leucine aminopepidase activity tended to be higher below the sewage treatment plant. The bacteriological parameters for River Słupia were compared with the relevant data for other river ecosystems.

Słowa kluczowe

EN

culturable proteolytic bacteria; leucine aminopeptidase; river

PL

aminopeptydaza leucynowa; bakterie proteolityczne; rzeka

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2009
• Tom: Vol. 57, nr 3
• Strony: 561--565
• Opis fizyczny: Bibliogr. 22 poz.,Rys., tab.,

Twórcy

autor

Skórczewski P.

autor

Mudryk Z.

• Department of Experimental Biology, Pomeranian Pedagogical University, Arciszewskiego 22B, 76-200 Słupsk, Poland,

Bibliografia

• 1. Ainsworth A.M., Goulder R. 1998 - Microbial organic-nitrogen transformations along the Swale - Ouse river system. Northern England - Sci. Tot. Environ. 210/211: 329-355.
• 2. Ainsworth A.M., Goulder R. 2000 - Downstream change in leucine aminopeptidase activity and leucine assimilation by epilithic microbiota along the River Swale, northern England - Sci. Tot. Environ. 251/252: 191-204.
• 3. Bellos D., Sawidis T., Tsekos I. 2004 - Nutrient chemistry of River Pinios (Thessalia, Greece) - Environ. Inter. 30: 105-115.
• 4. Brown S.E., Goulder R. 1999 - Change in reverine epilithic extracellular enzyme activity in response to fish farm effluent - Lett. Appl. Microbiol. 29: 385-388.
• 5. Chappell K.R., Goulder R.A. 1995 - A between river comparison of extracellular - enzyme activity - Microbial Ecol. 29: 1–17.
• 6. Chrost R.J. 1991 - Environmental control of the synthesis and activity of aquatic microbial ectoenzymes (In: Microbial enzymes in aquatic environments. Ed. R.J. Chrost) - Springer-Verlag, New York, pp. 29-59.
• 7. Ferrer E.B., Stapert E.M., Sokalski W.T. 1963 - A medium for improved recovery of bacteria from water - Can. J. Microbiol. 9: 420-422.
• 8. Foreman C.M., Franchini P., Sinsabaugh R.L. 1998 - The trophic dynamics of riverine bacterioplankton: Relationships among substrate availability, ectoenzyme kinetics, and growth - Limnol. Oceanogr. 43: 1344-1352.
• 9. Kang J.Y., Goulder R. 1996 - Epiphytic bacteria downstream of sewage-works outfalls - Wat. Res. 30: 501-510.
• 10. Keith S., Arnosti C. 2001 - Extracellular enzyme activity in a river-bay-shelf transect variations in polysaccharide hydrolysis rates with substrate and size class - Aquat. Microbiol. Ecol. 24: 243-253.
• 11. Kiersztyn B., Siuda W., Chrost R .J. 2002 - Microbial ectoenzyme activity: useful parameters for characterizing the trophic conditions of lakes - Pol. J. Environ. Stud. 11: 367-373
• 12. Małecka M., Donderski W. 2006 - Heterotrophic bacteria inhabiting water of the river Brda on the Bydgoszcz town section - Balt. Coast. Zone 10: 31-46.
• 13. Moczulska A., Antonowicz J., Krzyk K. 2006 - The influence of Słupsk agglomeration on the quality of the water of the River Słupia - Słup. Prace Biol. 3: 45-56. (in Polish)
• 14. Mokbel W.A., Yamakanamardi S.M. 2008 - Temporal variations in the abundance of heterotrophic bacteria in ground water according to land use patterns in Mysore district, India - Afric. J. Environ. Sci. Tech. 2: 59-67.
• 15. Mudr yk Z. 1981 - Occurrence of physiological groups bacteria inhabiting water of the River Słupia - Słup. Prace Mat-Przyr. 2: 113-124. (in Polish).
• 16. Mudryk Z., Donderski W. 1997 - The occurrence of hetrotrophic bacteria decomposing some macromolecular compounds in shallow estuarine lakes - Hydrobiologia, 342/343: 71-78.
• 17. Niewolak S. 1998 - The evaluation of the contamination degree and the sanitary and bacteriological state of the waters in the Czarna Hańcza river in the region of Suwałki and Wigry National Park - Pol. J. Environ. Stud. 7: 229-41.
• 18. Rosso A.L., Azam F. 1987 - Proteolytic activity in coastal oceanic waters: depth distribution and relationship to bacterial populations - Mar. Ecol. Prog. Ser. 41: 231-240.
• 19. Siebel E., Wang Y., Egli T., Hammes F. 2008 - Correlations between total cell concentration, total adenosine tri-phosphate concentration and heterotrophic counts during microbial monitoring of drinking water - Drin. Wat. Eng. Sci. Dis. 1: 71-86.
• 20. Wehr J.D., Petersen J., Findlay S. 1999 - Influence of three contrasting detritial carbon sources on planktonic bacterial metabolism in a mesotrophic lake - Microbial Ecol. 37: 23-35.
• 21. Wilczek S., Fischer H., Brunke M., Pusch M. T. 2004 - Microbial activity within a subaqueous dune in a large lowland river (River Elbe, Germany) - Aqua Microbiol. Ecol. 36: 83-97.
• 22. Worm J., Jensen L.E., Hansen T.S., Sondergaard M., Nybroe O. 2000 - Interactions between proteolytic and non-proteolytic Pseudomonas fluorescens affect protein degradation in a model community - FEMS Microbial Ecol. 32: 103-109.