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Polish Journal of Ecology

Tytuł artykułu

Phytoplankton extracellular phosphatases: Investigation using the ELF (enzyme labelled fluorescence) technique

Autorzy Strojsova, A.  Vrba, J. 
Treść / Zawartość
Warianty tytułu
Języki publikacji EN
EN Phosphorus deficiency is frequently the limiting factor in phytoplankton growth and the major source of selective pressure on single populations in a variety of aquatic environments. Phytoplankton species have several mechanisms that allow them to overcome phosphorus starvation, such as high-affinity uptake of orthophosphate (Pi), stand-by energy metabolism or utilization of phosphorus from stored polyphosphates. This short review is focused on the production of extracellular phosphatases that is usually accompanied by high-affinity uptake of Pi. Extracellular phosphatases are localized on thecell membrane, where they catalyze the liberation of Pi from various organic compounds that are too large to be directly transported into the cell. The production of extracellular phosphatases is not a general response to starvation in phytoplankton; it is a species-specific or cell-specific feature. The ELF (Enzyme Labelled Fluorescence) technique enables direct visualization of the sites of enzymatic activity and therefore gives detailed information about the physiological status of natural phytoplankton.
Słowa kluczowe
PL algi   fitoplankton   fosforan  
EN algae   cell-specific activity   cyanobacteria   ELF97 phosphate  
Wydawca Museum and Institute of Zoology, Polish Academy of Sciences
Czasopismo Polish Journal of Ecology
Rocznik 2006
Tom Vol. 54, nr 4
Strony 715--723
Opis fizyczny
autor Strojsova, A.
autor Vrba, J.
  • 1Biological Centre AS CR, Hydrobiological Institute, Na Sadkach 7, CZ.370 05 Ceske Budejovice, Czech Republic
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