Toxic Cyanobacteria strains in lowland dam resevoir (Sulejów Res., Central Poland): amplification of mcy genes for detection and identification

• Autorzy: Mankiewicz-Boczek Joanna , Urbaniak Magdalena , Romanowska-Duda Zdzisława , Izydorczyk Katarzyna
• Języki publikacji: EN

Abstrakty

EN

Excessive eutrophication causes the growth of microcystin-producing Cyanobacteria and leads to an increased human health risk. This paper reports the analyses of Cyanobacteria toxigenicity (the degree of potential toxicity) in lowland dam resevoir (Sulejów Resevoir, Central Poland) by the use of PCR-based methods. The resevoir (area 22 km2, mean depth 3.3 m) is very eutrophic water body permanently blooming with blue-green algae (mainly Microcystis aeruginosa). For identification of cyanobacterial genus the 16S rRNA region was used. Cyanobacterial genus was detected during the whole monitoring period in summer 2003. The potential toxicity of cyanobacteria was determinated by amplification of selected mcyA,B,E genes in the microcystin biosynthesis pathway. All of the analyzed genes were detected at the beginning of the growing season during low cyanobacterial biomass (0.67 mg 1^-1). 89% of the samples were found to be positive for mcyA detection. Early detection of mcy genes at the beginning of summer preceded a period of the highest microcystins concentration (2.91 [mi]g 1^-1 in maximum) and toxicity established by ELISA (enzyme-linked immunosorbent assay - enables determination of microcystins concentration) and PPIA (protein phosphatase inhibition assay - enables estimation of microcystins toxicity). We show that toxigenic (potentially toxic) strains of cyanobacteria occured in Sulejów Resevoir throughout the summer and genetic markers were effective in early identification of microcystin-producing genera. Application of molecular methods in parallel with toxicity testes can provide complete information to prevent any human healt risk.

Słowa kluczowe

EN

cyanobacteria; mcy gene cluster; microcystins; toxicity

PL

gen mcy; Sulejów zbiornik wodny; toksyczność

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2006
• Tom: Vol. 54, nr 2
• Strony: 171--180
• Opis fizyczny: Bibliogr. 41 poz.,Tab., wykr.,

Twórcy

autor

Mankiewicz-Boczek Joanna

• International Centre for Ecology, Polish Academy of Science, Tylna 3, 90-364, Łódź, Poland

autor

Urbaniak Magdalena

• International Centre for Ecology, Polish Academy of Science, Tylna 3, 90-364, Łódź, Poland

autor

Romanowska-Duda Zdzisława

• Department of Ecophysiology and Plant Development, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland

autor

Izydorczyk Katarzyna

• International Centre for Ecology, Polish Academy of Science, Tylna 3, 90-364, Łódź, Poland

• International Centre for Ecology, Polish Academy of Science, Tylna 3, 90-364 , Łódź, Poland,

Bibliografia

• Ambrożewski Z. 1980 – Monitoring Zbiornika wodnego Sulejów [Monitoring of Sulejów Reservoir] – Wydawnictwa Komunikacji i Łączności, Warszawa, Poland (in Polish).
• An J.S., Carmichael W.W. 1994 – Use a colorymetric protein phosphatase inhibition assay and enzyme linked immunosorbent assay for study of microcystins and nodularins - Toxicon, 32: 1495–1507.
• Baker J.A., Entsch B., Neilan B.A., McKay D.B. 2002 – Monitoring Changing Toxigenity of a Cyanobacterial Bloom by Molecular Methods – Appl. Environ. Microbiol., 68: 6070–6076.
• Bittencourt-Oliveira M. 2003 – Detection of potential microcystin-producing cyanobacteria in Brazilian reservoirs with a mcyB molecular marker – Harmful Algae, 2: 51–60.
• Briand J.F., Jacquet S., Bernard C., Humbert J.F. 2003 – Health hazards for terrestial vertebrates from toxic cyanobacteria in surface water ecosystems – Vet. Res. 34: 361–377.
• Carmichael W.W. 2001 – Heath Effects of Toin-Producing cyanobacteria: The Cyano-HABs – HERA, 7: 1393–1407.
• Chorus I., Bartram J. 1999 – Toxic Cyanobacteria in Water. A Guide to their Public Health Consequences, Monitoring and management - London: E.&F. N. Spon.
• Christiansen G., Fastner J., Erhard M., Borner T., Dittman E. 2003 – Microcystin Biosynthesis in Planktothrix: Genes, Evolution and Manipulation – J. Bacteriol. 185: 564–572.
• Codd G.A., Morrison L.F., Metcalf J.S. 2005 – Cyanobacterial toxins: risk management for health protection – Toxicol. Appl. Pharm. 203: 264–272.
• De Figueiredo D.R ., Azeiteiro U.M., Esteves S.M., Gonclaves F.J.M., Peteira M.J. 2004 – Microcystin-producing blooms - a serious global public healh issue – Ecotoxico. Environ. Safety, 59: 151–163.
• Dittmann E., Erhard M., Kaebernick M., Scheler Ch., Neilan B.A., von Döhren H., Börner Th. 2001 – Altered expression of two light-dependent genes in a microcystin-lacking mutant of Microcystis aeruginosa PCC 7806 – Microbiology, 147: 3113–3119.
• Errikson J.E., Hagerstrand H., Isomaa B. 1987 – Cell selective cytotoxicty of peptide toxin from the cyanobacterium Microcystis aeruginosa – Bioch. Bioph. Acta, 930: 304–310.
• Falconer I.R. 1994 – Health problems from exposure to cyanobacteria and proposed safety guidelines for drinking and recreational water (In: Detection methods for cyanobacterial toxins, Eds: G.A. Codd, T.M. Jefferies, C.W. Keevil, E. Potter) – The Royal Society of Chemistry, Cambridge, United Kingdom, pp. 3–10.
• Fleming L.E., Rivero C., Burns J., Williams Ch., Bean J.A., Shea K.A., Stinn J. 2002 – Blue green algal (cyanobacterial) toxins, surface drinking water, and liver cancer in Florida – Harmful Algae, 1: 157–168.
• Foulds I.V., Granacki A., Xiao C., Krull U.J., Castle A., Horgen P.A. 2002 – Quantification of microcystin-producing cyanobacteria and E. coli in water by 5’-nuclease PCR – J. Appl. Microbiol. 93: 825–834.
• Giovannoni S.J., DeLong E.F., Schmidt T.M., Pace N.R. 1990 – Tangential flow filtration and preliminary phylogenetic analysis of marine picoplankton – Appl. Environ. Microbiol. 568: 2572–2575.
• Golterman H.L., Clynio R.S., Ohstand M.A.M. 1988 – Methods for physical and chemical analysis of freshwater – IBP Hand Book.
• Hisbergues M., Christiansen G., Rouhiainen L., Sivonen K., Borner T. 2003 - PCR-based identification of microcysinproducing genotypes of different cyanobacterial genera – Arch. Microbiol. 180: 402–410.
• Izydorczyk K., Tarczyńska M., Jurczak T., Mrowczynski J., Zalewski M. 2005 - Measurment of Phycocyanin Fluorescence as an Online Early Warning System for Cyanobacteria in Reservoir Intake Water – Environ. Toxicol. 20: 425–430.
• Jurczak T., Tarczyńska M., Karlsson K., Meriluoto J. 2004 – Characterization and Diversity of Cyano-bacterial Hepatotoxins (Microcystins) in Blooms from Polish Freshwaters Identified by Liquid Chromatography-Electrospray Ionisation Mass Spectrometry - Chromatographia 59: 571–578.
• Kaebernick M., Dittmann E., Borner T., Neilan B.A. 2002 – Multiple Alternate Transcripts Direct the Biosynthesis of Microcystin, Cyanobacterial – Appl. Environ. Microbiol. 68: 440–455.
• Komárkova J., Vyhnalek V., Kubecka J. 1995 – Impact of fishstock manipulation on the composition of net phytoplankton in the Rimov Reservoir (Czech Republic) – Wat. Sci. Technol. 32: 207–216.
• Kurmayer R ., Dittmenn E., Fastner J., Chorus I. 2002 – Diversity of microcystin genes within a population of the toxic cyanobacterium Microcystis spp. In Lake Wannsee (Berlin, Germany) – Microbiol. Ecol. 43: 107–118.
• Kurmayer R., Kutzenberger T. 2003 Application of Real-Time PCR for Quantification of Microcystin Genotypes in a Population of the Toxic Cyanobacterium Microcystis sp. – Appl. Environ. Microbiol. 69: 6723–6730.
• Mankiewicz J., Komarkova J., Izydorczyk K., Jurczak T., Tarczyńska M., Zalewski M. 2005 – Hepatotoxic cyanobacterial blooms in lakes of North Poland – Environ. Toxicol. 20: 499–506.
• Mankiewicz J., Tarczyńska M., Walter Z., Zalewski M. 2003 – Natural toxins from Cyanobacteria – Acta Biol. Cracow. Series Botanica, 45: 9–20.
• OECD. 1983 – Eutrophication of water. Monitoring assessment and control – Technical Report. Environment Directorate, OECD, Paris. 154 pp.
• Oh H.-M., Lee S.J., Kim J.-H., Kim H.-S., Yoon B.-D. 2001 – Seasonal Variation and Indirect Monitoring of Microcystin concentrations in Daechung Reservoir, Korea - Appl. Environ. Microbiol. 67: 1484–1489.
• Pan H., Song L., Liu Y., Borner T. 2002 - Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples – Arch. Microbiol. 178: 421–427.
• Pawlik-Skowrońska B., Skowroński T., Pirszel J., Adamczyk A. 2004 – Relatrionship between cyanobacterial bloom composition and anatoxin-a and microcystin occurrence in the eutrophic dam reservoir (SE Poland) – Pol. J. Ecol. 52: 479–490.
• Rouhiainen L., Vakkilainen T., Siemer B.L., Buikema W., Haselkorn R., Sivonen K. 2004 – Genes Coding for Hepatotoxic Heptapeptides (Microcystins) in the Cyanobacterium Anabaena Strain 90 – Appl. Environ. Microbiol. 70: 686–692.
• Tarczyńska M., Izydorczyk K., Zalewski M. 2001a – Optimization of monitoring strategy for eutrophic reservoirs with toxic cyanobacterial blooms – Proceeding of 9th International Conference on the Conservation and Management of Lakes, Otsu, Japan. 3C/D-P83: 572–575.
• Tarczyńska M., Romanowska-Duda Z., Jurczak T., Zalewski M. 2001b – Toxic cyanobacterial blooms in a drinking water reservoirs – causes, consequences and management strategy – Wat. Sci. and Technol. 1: 237–246.
• Taton A., Grubisic S., Brambilla E., De Wit R., Wilmotte A. 2003 – Cyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a morphological and molecular approach – Appl. Environ. Microbiol. 69: 5157–5169.
• Sivonen K., Carmichael W.W., Namikoshi M., Rinchart K.L., Dablem A.M., Nicmela S.I. 1990 – Isolation and characterization of hepatotoxic microcystin homologs from the filamentous freshwater cyanobacterium Nostoc sp. strain 152 – Appl. Environ. Microbiol. 56: 2650–2657.
• Spoof L. 2004 – High-performance liquid chromatography of microcystins and nodularins, cyanobacterial peptide toxins – Doctoral Thesis. Åbo Academy University, Turku- Åbo, Finland.
• Vaitomaa J., Rantala A., Halinen K., Rouhiainen L., Tallberg P., Mokelke L., Sivonen K. 2003 – Quantitative Real-Time PCR for Determination of Microcystin Synthetse E Copy Numbers for Microcystis and Anabaena in Lakes – Appl. Environ. Microbiol. 69: 7289–7297.
• Via-Ordorika L., Fastner J., Kurmayer R., Hisbergues M., Dittmann E., Komarek J., Erhard M., Chorus I. 2004 - Distribution of Microcystin-Producing and Non-Microcystin-Producing Microcystis sp. In European Freshwater Bodies: Detection of Microcystins and Microcystin Genes in Individual Colonies – System. Appl. Microbiol. 27: 592–602.
• WHO 2003 – Guildlines for safe recreational water environments. Coastal and fresh waters - 1, pp.150.
• Wilmotte A. 1994 – Molecular evolution and taxonomy of the Cyanobacteria (In: Molecular Biology of Cyanobacteria, Ed.: D.A. Bryant) - Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 1–25.
• Zalewski M., Wagner I., Turczyńska M. 2000 – Ecohydrological approach for elimination of toxic algal blooms in lowland reservoir - Verh. Int. Ver. Limnol. 27: 1–8.