Enzymatic activity and degradation of organic macromolecules by neustonic and planktonic bacteria in an estuarine lake

• Autorzy: Mudryk Zbigniew Jan , Skórczewski Piotr
• Języki publikacji: EN

Abstrakty

EN

Neustonic (film layer -90 [mi]m, surface layer - 240 [mi]m) and planktonic bacteria (subsurface layer - 10-15 cm) participating in the processes of decomposition of organic macromolecular compounds, and their potential capability to synthesise extracellular enzymes were studied in a shallow estuarine lake (Lake Gardno - Baltic coast). The studied bacteria were capable of decomposing a wide spectrum of organic macromolecular compounds. Most bacteria inhabiting surface and subsurface water layers hydrolysed lipids, proteins and DNA. The microflora hydrolysing cellulose was represented by the least abundant group of organisms. Of the studied enzymes, alkaline and acid phosphatases, leucine arylaminase, esterase, and esterase lipase were synthesised most actively while [Beta]-glucouronidase ([Beta]Gl) and a-fucosidase ([alfa]Fu) were synthesized least actively. It can be clearly seen that enzymatic activity was stratified, and there were differences between studied water layers. Bacterial strains isolated from surface and subsurface layers were more active in synthesizing extracellular enzymes than bacteria inhabiting the film layer. Bacteria isolated from various parts of Lake Gardno synthesized the tested hydrolytic enzymes with a similar intensity.

Słowa kluczowe

EN

bacterioneuston; bacterioplancton; enzymatic activity; estuarine lake; organic matter decomposition

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2006
• Tom: Vol. 54, nr 1
• Strony: 3--14
• Opis fizyczny: Tab., wykr.,

Twórcy

autor

Mudryk Zbigniew Jan

• Department of Experimental Biology, Pomeranian Pedagogical University,Department of Experimental Biology, Pomeranian Pedagogical University,Department of Arciszewskiego 22 B, 76-200 Słupsk, Poland

autor

Skórczewski Piotr

• Department of Experimental Biology, Pomeranian Pedagogical University,Department of Experimental Biology, Pomeranian Pedagogical University,Department of Arciszewskiego 22 B, 76-200 Słupsk, Poland

• Department of Experimental Biology, Pedagogical University, Arciszewskiego str. 22, 76-200 Słupsk, Poland,

Bibliografia

• Albers C. S., Kattner G., Hagen W. 1996 – The compositions of wax esters, triacylglycerols and phospholipids in Arctic and Antarctic copepods: evidence of energetic adaptations – Mar. Chem. 55: 347–358.
• Arnosti C., Jørgensen B. B., Sagemann J., Tramdrup T. 1998 – Temperature dependence of microbial degradation of organic matter in marine sediment: polysaccharide hydrolysis, oxygen consumption, and sulfate reduction – Mar. Ecol. Prog. Ser. 165: 59–70.
• Austin C., Allen S. D., Mills A., Colwell R. 1977 – Numerical taxonomy of heavy metal- tolerant bacteria isolated from an estuary - Can. J. Microbiol. 23: 1433–1447.
• Billen G., Fontigny A. 1987 – Dynamics of a phaeocystis – dominated spring bloom in Belgian coastal waters, II. Bacterioplankton dynamics – Mar. Ecol. Prog. Ser. 37: 249–257.
• Boetius A. 1995 – Microbial hydrolytic enzyme activities in deep-sea sediments – Helgoland Mar. Res. 49: 177–187.
• Boschker H. T. S., Cappenberg T. E. 1998 – Patterns of extracellular enzyme activities in litorall sediments of Lake Gooimeer, The Netharlands – FEMS Microbiol. Ecol. 25: 79–86.
• Brown S. E., Goulder R. 1996 – Extracellular-enzyme activity in trout –farm effuents and recipient river – Aqua. Res. 27: 895–901.
• Chróst R., Overbeck J. 1987 – Kinetics of alkaline phosphatase activity and phosphorus availability for phytoplankton and bacterioplankton in lake Pluβsee (North German eutrophic lake) – Microbiol. Ecol. 13: 229–248.
• Chróst R., Gajewski A. 1995 – Microbial utilization of lipids in lake water – FEMS Microbiol. Ecol. 18: 45–50.
• Dahlbäck B., Gunnarsson L. A., Hermansson M., Kjelleberg S. 1982 – Microbial investigations of surface microlayers, water column, ice and sediment in the Arctic Ocean – Mar. Ecol. Prog. Ser. 9: 101–109.
• Daubner I. 1967 – Mikrobiologia vody. Slov. Akad. Vied. Bratyslava, 345 pp.
• Dell Anno A., Marreale D., Pusceddu A., Fabiano M., Danovaro R. 1999 – Particulate nucleic acids dynamics in a highly oligotrophic system: the Cretan Sea (Eastern Mediterranean) Mar. Ecol. Prog. Ser. 186: 19–30.
• De Flaun M. F., Paul J. H., Jeffrey W. H. 1987 – The distribution and molecular weight of DNA dissolved in subtropical estuarine and oceanic environments – Mar. Ecol. Prog. Ser. 33: 29–40.
• Dethier M. N. 1992 – Classifying marine and estuarine natural communities: An alternative to the coward in system – J. Nat. Areas 12: 90–100.
• Ferrer E. B., Stapert E. M., Sokalski W. T. 1963 – A medium for improved recovery of bacteria from water – Can. J. Microbiol. 9: 420–422.
• Gajewski A., Kirschner A. K., Velimirov B. 1997 – Bacterial lipolytic activity in a hypertrophic dead arm of the river Danube in Vienna – Hydrobiologia, 344: 1–10.
• Garrett W. D. 1965 – Collection of slick-forming materials from the sea surface – Limnol. Oceanogr. 10: 602–605.
• Harvey G. W., Burzell L. A. 1972 – A simple microlayer method for small samples - Limnol. Oceanogr. 17: 156–157.
• Hoppe H. G. 2003 – Phosphatase activity in the sea – Hydrobiologia, 493: 187–200.
• Hoppe H. G., Arnosti C., Herndel G. F. 2002 – Ecological significance of bacterial enzymes in marine environment (In: Microbial Enzymes in the Environment Activity, Ecology, and Applications. Eds. R. C. Burns, R. P. Dick) – Marcel Dekker, pp. 73–107.
• Hoppe H. G., Ulrich S. 1999 – Profiles of ectoenzymes in the Indian Ocean: phenomena of phosphatase activity in the mesopelagic zone – Aqua. Microbiol. Ecol. 19: 139–148.
• Jackson C. R., Foreman C. M., Sinsabaugh R. L. 1995 – Microbial enzyme activities as indicator of organic matter processing rates in a lake Erie coastal wetland – Freshwat. Biol. 34: 329–342.
• Jones S. E., Lock M. A. 1989 – Hydrolytic extracellular enzyme activity in heterotrophic biofilms from contrasting rivers – Freshwat. Biol. 22: 289–296.
• Jørgensen N. O., Kroer N., Coffin R. B., Lee C. 1993 – Dissolved free amino acids, combined amino acids, and DNA as source of carbon and nitrogen to marine bacteria – Mar. Ecol. Prog. Ser. 98: 135–148.
• Krstulović N., Solić M., 1988 – Distribution of proteolytic, amylolytic and lipolytic bacteria in the Kastela Bay – Acta Adria. 29: 75–82.
• Lackland D.T., Liu E. H., Koehler D. E., Chrzanowski T. 1982 – Cellulase activity as a measure of environmental perturbation in salt marsh ecosystems – Bot. Mar. 25: 143–148.
• MacCarthy M. D., Benner R., Hedges J. I. 1998 – Major bacterial contribution to marine dissolved organic nitrogen – Science, 281: 231–234.
• Maki J. S. 1993 – The air-water interface as an extreme environment (In: Aquatic Microbiology. An Ecological Approach. Ed. T. D Ford) - Blackwell Science Publications, Boston, Oxford, London, Edinburgh, Melbourne, Paris, Berlin, pp. 409–439.
• Mallet C., Debroas D. 1999 – Relations between organic matter and bacterial proteolytic activity in sediment surface layers of a eutrophic lake (Lake Aydat, Puy de Dôme, France) - Arch. Hydrobiol. 145: 39–56.
• Martinez J., Smith D.C., Steward D. F., Azam F. 1996 – Variability in ectohydrolytic enzyme actives of pelagic marine bacteria and its significance for substrate processing in the sea – Aqua. Microbial Ecol. 10: 223–230.
• Marxsen J., Schmidt H-H. 1993 – Extracellular phosphatase activity in sediments of the Breitenbach, a Central European mountain stream – Hydrobiologia, 253: 207–216.
• Meyer-Reil L. A. 1987 – Seasonal and spatial distribution on extracellular enzymatic activities and microbial incorporation of dissolved organic substrates in marine sediments Appl. Environ. Microbiol. 53: 1748–175.
• Middelboe M., Søndergaard A., Latarte Y., Boroch N.M. 1995 – Attached and free-living bacteria: production and polymer hydrolysis during a diatom bloom – Microbial Ecol. 29, 231–248.
• Mudryk Z. 2004 – Decomposition of organic and solubilization of inorganic phosphorus compounds by bacteria isolated from a marine sandy beach – Mar. Biol. 145: 1227–1234.
• Mudryk Z., Donderski W. 1997 – The occurrence of heterotrophic bacteria decomposing some macromolecular compounds in shallow estuarine lakes – Hydrobiologia, 342/343: 71–78.
• Mudryk Z., Skórczewski P. 2004 – Extracellular enzyme activity at the air-water inference of an estuarine lake – Estuar. Coast. Shelf Sci. 59: 59–67.
• Mudryk Z., Trojanowski J., Skórczewski P., Antonowicz J. 2003 – Chemical and bacteriological studies of surface and subsurface water layers in estuarine Lake Gradno Pol. J. Environ. Stud. 12: 199–206.
• Münster U., Chróst R. 1990 Organic composition and microbial utilization of dissolved organic matter (In: Aquatic Microbial Ecology. Biochemical and Molecular Approaches. Eds. J. Overbeck, R. Chróst) – Springer-Verlag, New York, Berlin, Heidelberg, Paris, Tokyo, Hong Kong, Barcelona, pp. 8–46.
• Münster U., Heikkinen E., Knulst J. 1998 - Nutrient composition, microbial biomass and activity at the air-water interface of small boreal forest lakes – Hydrobiologia, 363: 261–270.
• Quemeneur M., Marty Y. 1992 – Sewage influence in a macrotidal estuary: fatty acid and sterol distribution – Estuar. Coast. Shelf Sci. 34: 347–363.
• Patel A. B., Fukami K., Nishijama T. 2000 – Regulation of seasonal variability of aminopeptidase activities in surface and bottom waters of Uranouchi Inlet, Japan – Aqua. Microbial Ecol. 21: 139–149.
• Paul J. H., DeFlaun M. F., Jeefrey W. H., David A. W. 1988 – Seasonal and diel variability in dissolved DNA and in microbial biomass and activity in a subtropical estuary – Appl. Environ. Microbiol. 54: 718–727.
• Petrycka H., Mrozowska J., Kasza H. 1990 – Changes in bacterial microflora against the background of increasing eutrophication Goczałkowice Reservoir, southern Poland Acta Hydrobiol. 32: 55–66.
• Podgórska B., Mudryk Z. 2003 – Distribution and enzymatic activity of heterotrophic bacteria decomposing selected macromolecular compounds in a Baltic Sea beach – Estuar. Coast. Shelf Sci. 56: 539–546.
• Shiah F. K., Chen T. Y., Gong C. Ch., Chen Ch., Ciang K. P., Hung J. J. 2001 - Differential coupling of bacterial and primary production in mesotrophic and oligotrophic systems of the east China Sea – Aqua. Microbial Ecol. 23: 273–282.
• Sieburth J. N. 1978 – Bacterioplankton: nature, biomass, activity and relationship to the protist plankton – J. Phycol. 14: 31–41.
• Stewart G. J., Sinigalliano C. D., Garko O. 1991 – Binding of exogenous DNA to marine sediments and the effect of DNA sediment binding on natural transformation of Pseudomonas stutzeri strain ZoBell in sediment columns – Microbial Ecol. 85: 1–8.
• Strzelecki J., Półtorak T. 1971 – Coastal plankton lake Gardno during summer season Acta Hydrobiol. 13: 269–294.
• Sugita H., Oshima K., Fushion T., Deguchio Y. 1987 – Substrate specificity of heterotrophic bacteria in the water and sediment of carp culture pond – Aquaculture, 64: 39–46.
• Thompson A. J., Sinsabaugh R. L. 2000 - Matric and particulate phosphatase and aminopeptidase activity in limnetic biofilms Aqua. Microbial Ecol. 21: 151–159.
• Unanue M., Ayo B., Agis M., Slezak D., Herndl G. J., Iriberri J. 1999 – Ectoenzymatic activity and uptake of monomers in marine bacterioplankton described by a biphasic kinetic model – Microbial Ecol. 37: 36–48.
• Zdanowski M. K., Donachie S. P. 1993 – Bacteria in the sea-ice zone between Elephant Island and the South Orkneys during the Polish sea-ice zone expedition, (December 1988 to January 1989) – Polar Biol. 13: 245–254.
• Zdanowski M. K., Figuerias F. G. 1999 – CFU bacterial fraction in the estuarine upwelling ecosystem of Ria de Vigo, Spain: variability in abundance and their ecophysiological description – Mar. Ecol. Prog. Ser. 182: 1–15.