The chromatographic behavior of wild type and pegylated lysozyme on reversed phase-high performance liquid chromatoraphy media

• Autorzy: Schöbel J. , Przybycien T. M. , Büttner H.
• Konferencja: Międzynarodowa Konferencja Młodych Naukowców "Środowisko, Rozwój, Inżynieria, Modelowanie" (5 ; 2004 ; Kraków)
• Języki publikacji: EN

Abstrakty

EN

The chromatographic behavior of wild type lysozyme and PEGylated lysozyme on silica-based C4 and C18 reversed phase media using and HPLC system was studied. We studied the effects of medias. alkyl chain length on wild type and modified lysozyme. The impact of a covalently bonded PEG chain on the protein.s retention behavior was examined. Using two different reversed phase media we did not observe different elution profiles; adsorption is not influenced by medias. alkyl chain length. According to prior work, the strongly retained wild type lysozyme was structurally perturbed due to adsorption. The elution profile of PEGylated lysozyme showed an even much stronger retained peak fraction, which indicates that PEG has stronger interactions with the hydrophobic stationary phase.

Słowa kluczowe

EN

chromatographic behavior; lysozyme; PEGylated lysozyme

• Wydawca: Wydawnictwo Politechniki Krakowskiej im. Tadeusza Kościuszki
• Czasopismo: Czasopismo Techniczne. Środowisko
• Rocznik: 2004
• Tom: R. 101, z. 9-Ś
• Strony: 77--85
• Opis fizyczny: Bibliogr. 11 poz., wykr.

Twórcy

autor

Schöbel J.

• Fachhochschule Münster, Germany

autor

Przybycien T. M.

• Fachhochschule Münster, Germany

autor

Büttner H.

• Fachhochschule Münster, Germany

Bibliografia

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