Modelowanie inaktywacji termicznej glukoamylazy z aspergillus niger

• Autorzy: Bryjak J.

Warianty tytułu

EN

Thermal inactivation kinetics of aspergillus niger glucoamylase

• Języki publikacji: PL

Abstrakty

PL

Inaktywację termiczną glukoamylazy z Aspergillus niger zaliczono do procesu o charakterze dwufazowym na podstawie analizy zależności logarytmu aktywności (szybkości reakcji) od czasu reakcji. Spośród pięciu zanalizowanych modeli matematycznych dwa o charakterze ogólnym prawidłowo odwzorowywały zmiany aktywności enzymu pod wpływem podwyższonej temperatury: model równoczesnej inaktywacji, zakładający występowanie dwóch form izoenzymatycznych oraz dwa modele zgodne z mechanizmem nieodwracalnej inaktywacji następczej, zakładające występowanie zależnych od temperatury pośrednich form enzymu o mniejszej aktywności. Wybór między trzema modelami oparto na analizie zależności wartości stałych równań kinetycznych od temperatury oraz analizie prawdopodobnego składu formy pośredniej enzymu.

EN

Aspergillus niger glucoamylase undergoes complex thermal inactivation phenomena, as revealed by the two-slope pattern of the enzyme logarithmic activity versus time curves. Two proposed general inactivation models are shown to fit enzyme inactivation data reasonably well. They are as follows: parallel isozyme model which assumes the presence of two different active forms of glucoamylase and two models of inactivation by a series mechanism, involves intermediate, temperature dependent and less active forms of the enzyme. Discrimination between these models was based on dependence of kinetic constants on temperature as well as the analysis of possible structure of the enzyme intermediate forms.

Słowa kluczowe

PL

chemia; glukoamylaza; inaktywacja termiczna

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Inżynieria Chemiczna i Procesowa
• Rocznik: 2001
• Tom: T. 22, z. 2
• Strony: 345--362
• Opis fizyczny: Bibliogr. 53 poz., tab., rys.

Twórcy

autor

Bryjak J.

• Instytut Inżynierii Chemicznej i Procesowej Politechniki Wrocławskiej

Bibliografia

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