Scale-up of slow rotary countercurrent chromatographic isolation of glucoraphanin and glucoraphenin from broccoli seeds and radish seeds

• Autorzy: Fu Y. , Du Q. , Wang K.
• Języki publikacji: EN

Abstrakty

EN

A scaled-up SRCCC apparatus equipped with a 40-L column was constructed on the basis of separations of crude broccoli seed extract and crude radish seed extract using a conventional SRCCC instrument. Scaled-up separation of 500 g of crude broccoli seed extract with n -butanol-acetonitrile-10% (NH 4 ) 2 SO 4 aqueous solution 1:0.5:2 (υ/υ) as solvent system yielded 61.5 g glucoraphanin product of purity 91.2%. Separation of 500 g crude radish seed extract with the same solvents in the ratio 0.5:1:2 (υ/υ) afforded 26.7 g glucoraphenin product of purity 94.5%. Recovery of glucoraphanin and glucoraphenin from the crude extracts was 98.3 and 98.9%, respectively. The results demonstrated that this SRCCC technology is useful for separation of glucosinolates from plant extracts.

Słowa kluczowe

EN

slow rotary countercurrent chromatography; glucoraphanin; glucoraphenin; radish seeds; broccoli seeds; scale-up

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 4
• Strony: 697--707
• Opis fizyczny: Bibliogr. 23 poz., rys., tab.

Twórcy

autor

Fu Y.

autor

Du Q.

autor

Wang K.

• Zhejiang Gongshang University Institute of Food and Biological Engineering Hangzhou 310035 China,

Bibliografia

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