Specific separation of thiamine from hydrophilic vitamins with aqueous dioxane on precoated silica TLC plates

• Autorzy: Mohammad A. , Zehra A.
• Języki publikacji: EN

Abstrakty

EN

Specific separation of thiamine hydrochloride from riboflavin, nicotinic acid, calcium D-pantothenate, pyridoxine hydrochloride, cyanocobalamin, and ascorbic acid has been achieved on commercial precoated silica gel 60F ₂₅₄ TLC plates with dioxane-water 1:1 (υ/υ ) as mobile phase. The spots were visualized under UV light. The effect of impurities (metal cations and inorganic anions) on the chromatography of thiamine hydrochloride was examined. The detection limit for thiamine hydrochloride was 0.09 µg per spot and the relative standard deviation of the R _F value of thiamine hydrochloride in five analyses was 14.99%. The applicability of the method to the identification of thiamine in pharmaceutical formulations was also tested.

Słowa kluczowe

EN

separation; hydrophilic vitamins; aqueous dioxane; precoated silica TLC plates

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 4
• Strony: 637--642
• Opis fizyczny: Bibliogr. 17 poz., rys., tab.

Twórcy

autor

Mohammad A.

autor

Zehra A.

• Aligarh Muslim University Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology Aligarh India,

Bibliografia

• [1] Pyka, J. Sliwiok, and A. Niestroj, Acta Polon. Pharm., 60 , 327 (2003)
• [2] A. Pyka, Chromatogr. Sci. Ser., 89 , 671 (2003)
• [3] F. Watanabe and E. Miyamoto, Chromatogr. Sci. Ser., 89 , 589 (2003)
• [4] E.L. Ponder and J. Sherma, Acta Universitatis Cibiniensis, Seria F: Chemia, 7 , 3 (2004)
• [5] I. Baranowska and A. Kadziolka, Acta Chromatogr., 6 , 61 (1996)
• [6] E.L. Ponder, B. Fried, and J. Sherma, Acta Chromatogr., 14 , 70 (2004)
• [7] A.N. Diaz, A.G. Paniagua, and F.G. Sanchez, J. Chromatogr., 655 , 39 (1993)
• [8] R. Bhushan and M. Arora, National Acad. Sci. Lett., 25 , 159 (2002)
• [9] K. Tyrpien, R.R. Schefer, S. Bachmann, and K. Albert, J. Planar Chromatogr., 16 , 256 (2003)
• [10] N.U. Perisic-Janjic, M.R. Popovic, and T.Lj. Djakovic, Acta Chromatogr., 5 , 144 (1995)
• [11] V.M. Vostokov and V.R. Kartashov, Khimiya i Khimicheskaya Tekhnologiya, 49 , 115 (2006)
• [12] B. Srinivas and K. Srinivasulu, J. Indian Chem. Soc., 69 , 669 (1992)
• [13] R. Bhushan and V. Parshad, Biomed. Chromatogr., 8 , 196 (1994)
• [14] J. Sliwiok, A. Podgorny, A. Siwek, and B. Witkowska, J. Planar Chromatogr., 3 , 429 (1990)
• [15] J. Sliwiok, B. Kocjan, B. Labe, A. Kozera, and J. Zalejska, J. Planar Chromatogr., 6 , 492 (1993)
• [16] A. Pyka and W. Klimczok, J. Planar Chromatogr., 18 , 300 (2005)
• [17] N. Perisic-Janjic, S. Petrovic, and P. Hadzic, Chromatographia, 9 , 130 (1976).