Simultaneous densitometric TLC analysis of aceclofenac, paracetamol, and chlorzoxazone in tablets

• Autorzy: Mahajan V. K. , Bari S. B. , Shirkhedkar A. A. , Surana S. J.
• Języki publikacji: EN

Abstrakty

EN

A new simple, accurate, precise, rapid, and selective densitometric TLC method has been established for simultaneous analysis of aceclofenac, paracetamol, and chlorzoxazone in tablets. Identification and quantification were performed on 10 cm × 10 cm aluminium-backed TLC plates coated with 0.2 mm layers of silica gel 60 F 254 , previously washed with methanol, and using toluene-2-propanol-ammonia 4:4:0.4 (υ/υ) as mobile phase. Detection was performed at 274 nm. The R F values of aceclofenac, paracetamol, and chlorzoxazone were 0.28 š 0.01, 0.72 š 0.02, and 0.51 š 0.02, respectively. Calibration plots were linear in the range 400-1400 ng per band for aceclofenac, 2000-7000 ng per band for paracetamol, and 1000-3500 ng per band for chlorzoxazone, with correlation coefficients, r , 0.9995, 0.9993, and 0.9996, respectively. Recovery of aceclofenac, paracetamol, and chlorzoxazone were 99.54-100.44, 100.02-100.47, and 99.39-99.84%, respectively. The suitability of densitometric TLC for quantitative analysis of these compounds was proved by validation in accordance with the requirements of ICH Guidelines.

Słowa kluczowe

EN

aceclofenac; paracetamol; chlorzoxazone; TLC-densitometry; validation

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 4
• Strony: 625--636
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Mahajan V. K.

autor

Bari S. B.

autor

Shirkhedkar A. A.

autor

Surana S. J.

• R.C. Patel College of Pharmacy Shirpur, Dist: Dhule (M.S.) 425 405 India,

Bibliografia

• [1] J.O. Neil (Ed.) The Merck Index, 14th edn, Merck, White House Station, NJ, USA, 2006, p. 5
• [2] Martindale — The Complete Drug Reference, 35th edn, Pharmaceutical Press, London, 2007, p. 2
• [3] British Pharmacopoeia, International edn, Vol. 1, HMSO, Cambridge, 2005, p. 40
• [4] Indian Pharmacopoeia, Government of India, Ministry of Health and Family Welfare, Delhi, 1996, p. 554
• [5] H.P. Rang and M.M. Dale, Pharmacology, 5th edn, Churchill Livingstone, 2003, p. 251
• [6] United States Pharmacopeia — The National Formulary (USP28/NF 23), United States Pharmacopeial Convention, Rockville, MD, 2004, p. 2749
• [7] J.T. Stewart and K. Florey, Analytical Profiles of Drug Substances, Vol. 16, Academic Press, 2005, p. 119
• [8] P.R. Mahaparale, N. Sangshetti, and B.S. Kuchekar, Indian J. Pharm. Sci., 69 (2), 289 (2007)
• [9] N.M. EI Kousy, J. Pharm. Biomed. Anal., 20 , 185 (1999)
• [10] G. Garg, S. Saraf, and S. Saraf, Indian J. Pharm. Sci., 69 (5), 692 (2007)
• [11] I. Singhvi and A. Goyal, Indian J. Pharm. Sci., 69 (1), 145 (2007)
• [12] H.S. Lee, C.K. Jeong, S.J. Choi, S.B. Kim, M.H. Lee, G.I. Ko, and D.H. Sohn, J. Pharm. Biomed. Anal., 23 , 275 (2000)
• [13] R.K. Raja, G.G. Sankar, A.L. Rao, and R.J.V.L.N. Seshangiri, Indian Drugs, 42 , 693 (2005)
• [14] P.S. Selven and R. Gopinath, Asian J. Chem., 19 (2), 1004 (2007)
• [15] Y.S. El-Saharty, M. Refaat, and S.Z. el-Khateeb, Drug Dev. Int. Pharm., 28 , 571 (2002)
• [16] M.S. Bhatia, S.G. Kaskhedikar, and S.C. Chaturvedi, Indian Drugs, 34 (3), 149 (1997)
• [17] M. Gandimathi, T.K. Ravi, N. Shukla, and G. Sowmiya, Indian J. Pharm. Sci., 69 (1), 145 (2007)
• [18] J.L. Chawla, R.A. Sodhi, and R.T. Sane, Indian Drugs, 33 (4), 171 (1996)
• [19] A.F. Lavorante, C.K. Pires, and B.F. Reis, J. Pharm. Biomed. Anal., 42 , 423 (2006)
• [20] K. Kachrimanis, D.E. Braun, and U.J. Griesser, J. Pharm. Biomed. Anal., 43 , 407 (2007)
• [21] A. Panusa, G. Multari, G. Incarnato, and L. Gagliard, J. Pharm. Biomed. Anal., 43 , 1221 (2007)
• [22] M. Puranik, A. Hirudkar, S.J. Wadher, and P.G. Yeole, Indian J. Pharm. Sci., 68 (6), 737 (2006)
• [23] S.S. Zarapkar, N.P. Bhandari, and U.P. Halkar, Indian Drugs, 37 (10), 469 (2000)
• [24] S.S. Zarapkar, S.S. Kolte, A.A. Dhanvate, and S.A. Shivalkar, Indian Drugs, 33 (6), 275 (1996)
• [25] R.A. Sodhi, J.L. Chawla, and R.T. Sane, Indian Drugs, 33 (6), 280 (1996)
• [26] E. Tanaka, J. Pharm. Biomed. Anal., 16 , 899 (1998)
• [27] L.I. Bebawy and N.M. EI-Kousy, J. Pharm. Biomed. Anal., 20 , 663 (1999)
• [28] A.A. EI Bayoumi and S.M. Amer, J. Pharm. Biomed. Anal., 20 , 727 (1999)
• [29] ICH guidelines Q2A, Validation of Analytical Procedures — Methodology, 1994
• [30] Reviewer Guidance, Validation of Chromatographic Methods, 1994
• [31] ICH Guidelines Q2B, Validation of Analytical Procedures — Methodology, 1996.