Microemulsion liquid chromatographic screening of Simvastatin and its active metabolite in human plasma

• Autorzy: Malenovic A. , Jancic-Stojanovic B. , Medenica M. , Ivanovic D.
• Języki publikacji: EN

Abstrakty

EN

This paper reports development and validation of a new microemulsion liquid chromatographic (MELC) method for rapid screening of simvastatin and simvastatin acid in human plasma. Plasma samples were injected directly into the HPLC system after appropriate sample dilution with mobile phase. Separations were performed on a 4.6 mm × 150 mm, 5-µm particle, C 18 column, with UV detection at 238 nm. The mobile phase was 0.5% ( w/u ) diisopropyl ether, 1.0% ( w/u ) sodium dodecylsulphate (SDS), 4.0% ( w/v ) n -butanol, and 94.5% ( w/w ) aqueous 25 mM disodium hydrogen phosphate, pH 7.0, at a flow rate of 1 mL min ^-1 . The method was evaluated according to criteria stated in FDA bioanalytical method validation guidance. The unique approach applied in this paper enables direct analysis of simvastatin and simvastatin acid, so the method can be used to obtain reliable results in a rapid and simple way.

Słowa kluczowe

EN

microemulsion liquid chromatography; human plasma; simvastatin; active metabolite

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 4
• Strony: 595--607
• Opis fizyczny: Bibliogr. 16 poz., rys., tab.

Twórcy

autor

Malenovic A.

autor

Jancic-Stojanovic B.

autor

Medenica M.

autor

Ivanovic D.

• Faculty of Pharmacy Department of Drug Analysis Vojvode Stepe 450 Belgrade Serbia Faculty of Pharmacy Department of Physical Chemistry Vojvode Stepe 450 Belgrade Serbia,

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