Determination of the lipophilicity of selected isoquinoline alkaloids by RP-TLC

• Autorzy: Waksmundzka-Hajnos M. , Matosiuk D. , Petruczynik A. , Kijkowska-Murak U.
• Języki publikacji: EN

Abstrakty

EN

Nine alkaloids were chromatographed by reversed phase (RP) TLC on RP-18 with aqueous acetone or aqueous dioxane mobile phases. A variety of mobile phase additives (ammonia, diethylamine (DEA), tetrabutylammonium chloride (TBA-Cl)) were used to suppress ionization of the alkaloids and/or reduce ionic interactions with surface silanol groups. Ion-pair (IP) RP-TLC was also performed with aqueous acetone mobile phases and reagents such as pentane sulphonic acid (PSA), octane sulphonic acid (OSA), or di-(2-ethylhexyl)orthophosphoric acid (HDEHP) as mobile phase additives. In these systems retention of the alkaloids was determined using mobile phases with different modifier concentrations. Relationships between R _M and modifier concentration were sought. A linear semilogarithmic equation was fitted to experimental data and used to obtain lipophilicity values R _MW ( R _M for pure water), the slope ( S ), and φ 0 , the intercept with the x -axis. The retention of standards with known lipophilicity log P was then determined using the chromatographic systems described above and R _MW values were calculated. From these experimental data equations relating log P and R _MW were created for each system separately and these equations were used to estimate log P _exp values for the alkaloids. log P _exp , slope ( S ), and φ 0 values obtained by use of different TLC systems were also correlated.

Słowa kluczowe

EN

RP-TLC; hydrophobicity; isoquinoline alkaloids

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 4
• Strony: 563--573
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Waksmundzka-Hajnos M.

autor

Matosiuk D.

autor

Petruczynik A.

autor

Kijkowska-Murak U.

• Medical University of Lublin Department of Inorganic Chemistry 20-081 Lublin Poland Medical University of Lublin Department of Synthesis and Chemical Technology of Pharmaceutical Substances 20-081 Lublin Poland,

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