Computer-assisted optimization of liquid-liquid extraction for HPLC analysis of domperidone and pantoprazole in human plasma

• Autorzy: Sivakumar T. , Manavalan R. , Valliappan K.
• Języki publikacji: EN

Abstrakty

EN

This paper deals with optimization of a liquid-liquid extraction procedure for simultaneous HPLC analysis of domperidone and pantoprazole in human plasma. Central composite design and Derringer’s desirability function were used to optimize the concentration of KOH and the volume of ethyl acetate as the main factors affecting the liquid-liquid extraction procedure. After extraction, the analytes were separated quantitatively on a C 18 column with 10 mM pH 7.0 phosphate buffer-methanol-acetonitrile 48.46:20:31.54 ( υ / υ ) as mobile phase at a flow rate of 1.20 mL min ^-1 and with UV detection at 285 nm. It was concluded that extraction recovery of both the analytes was affected by KOH concentration and that recovery of pantoprazole was affected by ethyl acetate (extraction solvent) volume. Extraction recovery under optimum extraction conditions was 93.52% for domperidone and 92.72% for pantoprazole. The optimized extraction method was validated. Linearity was established for six levels in the ranges 10–1000 ng mL ^-1 for pantoprazole and 15–1000 ng mL ^-1 for domperidone. The lower limit of quantitation (LLOQ) and detection (LOD) were estimated as 9.84 and 5.91 ng mL ^-1 , respectively, for pantoprazole and 14.56 and 8.79 ng mL ^-1 for domperidone. The optimized method was linear, specific, accurate, and precise; the high recovery (>92%) and low relative standard deviation (<2.5%) enable reliable quantification of these analytes in spiked human plasma.

Słowa kluczowe

EN

pantoprazole; domperidone; liquid-liquid extraction; HPLC; central composite design; Derringer's desirability function

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 4
• Strony: 549--562
• Opis fizyczny: Bibliogr. 27 poz., rys., tab.

Twórcy

autor

Sivakumar T.

autor

Manavalan R.

autor

Valliappan K.

• Annamalai University Department of Pharmacy, Faculty of Engineering and Technology Annamalainagar, Tamil Nadu 608 002 India,

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