Isolation of glucosinolates from broccoli seeds by use of high-speed countercurrent chromatography

• Autorzy: Du Q. , Zhang L. , Cui H. , Wang K. , Xiu L. , Sheng L.
• Języki publikacji: EN

Abstrakty

EN

Cleavage of glucosinolates with myrosinase yields thioglycosidic compounds which have cancer chemoprevention activity. In this paper, glucosinolates in an extract (2.0 g) of broccoli seeds ( Brassica oleracea var. italica ) were separated by high-speed countercurrent chromatography (HSCCC) with the solvent system n -butanol-acetonitrile-10% ammonium sulfate solution 1:0.5:2.2 (v/v) to yield five glucosinolate compounds after desalting and decolorizing by MCI column chromatography. The five compounds, 7-methylsulfinylheptyl glucosinolate (22.4 mg), 4-pentenyl glucosinolate (33.6 mg), 3-butenyl glucosinolate (24.0 mg), 4-methylsulfinylbutyl glucosinolate (161.4 mg), and 3-methylsulfinylpropyl glucosinolate (29.6 mg), were identified by ESI-MS, ¹ H NMR and ¹³ C NMR. The purity of the products was >98%, and 7-methylsulfinylheptyl glucosinolate was obtained from broccoli seeds for the first time.

Słowa kluczowe

EN

broccoli seeds; glucosinolates; isolation; high-speed countercurrent chromatography

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 3
• Strony: 489--496
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Du Q.

autor

Zhang L.

autor

Cui H.

autor

Wang K.

autor

Xiu L.

autor

Sheng L.

• Zhejiang Gongshang University Institute of Food and Biological Engineering Hangzhou 310035 China,

Bibliografia

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