Analysis of lovastatin in human plasma by liquid chromatography coupled with tandem mass spectrometry

• Autorzy: Yu X. R. , Song M. , Hang T. J. , Wen A. D.
• Języki publikacji: EN

Abstrakty

EN

A sensitive and selective liquid chromatographic tandem mass spectrometric (LC-MS-MS) method for analysis of lovastatin in human plasma has been developed and validated. Ethyl acetate extraction was used for plasma sample preparation and simvastatin was used as internal standard. Chromatographic separation was achieved on a C ₁₈ column by isocratic elution with 83:17:0.1 ( v/v ) methanol-2 µM aqueous sodium acetate-formic acid as mobile phase, delivered at 1.0 mL min ^-1. MS-MS detection was performed using positive electrospray ionization and multiple-reaction monitoring with argon for collision-induced dissociation. Calibration plots were generated over the concentration range 0.05 to 20 ng mL ^-1 (r > 0.999) with a lower limit of quantification (LLOQ) of 0.05 ng mL ^-1. Intra and inter-day precision and accuracy were determined at four different concentrations, 0.05, 0.5, 2.0, and 10.0 ng mL ^-1, and precision ranged from 0.4 to 11.4% with the deviation always less than 15% ( n = 5). Extraction recoveries were from 86.8 to 94.1% for lovastatin and approximately 88.0% for simvastatin. The validated method was successfully applied to a bioequivalence study of two lovastatin tablets in 20 healthy volunteers.

Słowa kluczowe

EN

lovastatin; LC-MS/MS; human plasma; bioequivalence

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 3
• Strony: 399--410
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Yu X. R.

autor

Song M.

autor

Hang T. J.

autor

Wen A. D.

• China Pharmaceutical University Department of Pharmaceutical Analysis Jiangsu Nanjing 210009 China Forth Military Medical University of PLA Institute of Clinical Pharmacology Shangxi Xi'an 710032 China,

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