Thin-layer chromatographic analysis of psoralen in babchi ( Psoralea corylifolia ) oil

• Autorzy: Ali J. , Akhtar N. , Sultana Y. , Baboota S. , Ahmad S.
• Języki publikacji: EN

Abstrakty

EN

A simple, selective, precise, accurate, and cost-effective thin-layer chromatographic (TLC) method for analysis of psoralen in different brands of babchi (Psoralea corylifolia) oil has been developed and validated. Aluminium TLC plates precoated with silica gel 60F₂₅₄ were used as the stationary phase and n-hexane-acetone-formic acid 2:1:0.025 (v/v) as mobile phase. A compact, resolved psoralen peak (R_Fvalue 0.32 š 0.02) was observed by densitometric analysis in absorbance mode at 250 nm. Calibration data revealed a good linear relationship (r² = 0.9956) between peak area and concentration in the range 20-200 ng per spot. Mean š SD values of slope and intercept were 11.35 š 0.36 and 14.64 š 0.31, respectively. Statistical analysis proved the method to be a repeatable, selective, and accurate means of estimation of psoralen in different brands of babchi oil.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 2
• Strony: 277--282
• Opis fizyczny: Bibliogr. 9 poz., rys., tab.

Twórcy

autor

Ali J.

autor

Akhtar N.

autor

Sultana Y.

autor

Baboota S.

autor

Ahmad S.

• Jamia Hamdard University Department of Pharmaceutics, Faculty of Pharmacy New Delhi 110062 India Jamia Hamdard University Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy New Delhi 110062 India,

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