Separation of two main triterpene glycosides from sea cucumber Pearsonothuria graeffei by high-speed countercurrent chromatography

• Autorzy: Dong P. , Xue C. H. , Du Q. Z.
• Języki publikacji: EN

Abstrakty

EN

Holostane series glycosides have been isolated by a new and effective method. Two main triterpene glycosides from sea cucumber Pearsonothuria graeffei extract were successfully purified by one-step high-speed countercurrent chromatography (HSCCC) using a two-phase solvent systems comprising ethyl acetate-n-butanol- methanol-water 2.4:2.6:0.2:4.8 (v/v) to yield holothurin A and echinoside A with purities 93.5 and 95.5%, respectively. The chemical structures were elucidated by ESIMS and NMR analysis.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 2
• Strony: 269--276
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Dong P.

autor

Xue C. H.

autor

Du Q. Z.

• Ocean University of China College of Food Science and Technology No. 5, Yu Shan Road Qingdao Shandong Province 266003 P.R. China Zhejiang Congshang University Institute of Food and Biological Engineering Hangzhou 310035 P.R. China,

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