New application of mixed C₁₈ -SCX SPE clean-up in gas chromatographic-mass spectrometric determination of six β ₂ -agonist residues in pig's liver

• Autorzy: Shan J. , Pan C. , Zhang J. , Niu W.
• Języki publikacji: EN

Abstrakty

EN

A new mixed C₁₈-SCX SPE column and gas chromatography-mass spectrometry have been used for determination of six β 2-agonists residues in pig's liver. After hydrolysis, the six β 2-agonists were extracted with 6:4 (v/v) ethyl acetate-isopropanol and then cleaned on a commercial mixed-adsorbent solid-phase extraction column. The eluted solution was analyzed by gas chromatography-mass spectrometry (GC-MS) and quantified by the external standard method. Sample pre-treatment and GC-MS conditions were critically examined. The linear range was 0.005-2.00 mg L^-1 and correlation coefficients were 0.9987-1.000. The LOD of mabuterol, clenbuteroland, and salbutamol was 0.2 µg kg ^-1. Average recoveries of these three compounds from spiked pig's liver ranged from 65.3 to 95.8% and relative standard deviations were between 5.4 and 9.9%. The LOD of cimaterol, bromobuterol, and ractopamine was 0.3 µg kg ^-1. Average recoveries of these three compounds from spiked pig's liver ranged from 63.8 to 88.1% and relative standard deviations were between 5.0 and 9.7%. These results indicate the method is highly sensitive, simple, inexpensive, and results in good purification. It could also meet requirements for analysis of pig's urine in domestic and import/ export inspection work.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2008
• Tom: Vol. 20, no. 1
• Strony: 43--57
• Opis fizyczny: Bibliogr. 22 poz., rys., tab.

Twórcy

autor

Shan J.

autor

Pan C.

autor

Zhang J.

autor

Niu W.

• College of Science, China Agricultural University, Beijing 100094, China Quality Control and Inspection Center for Domestic Animal Products, MOA, 100026, Beijing, China Disease Control Center of Wuxi City, Wuxi 214023, China

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