An adsorption of bovine serum albumin on carbon/zirconium oxide microfiltration membranes at different pH’s as determined from breakthrough curves

• Autorzy: Białopiotrowicz T. , Blanpain P. , René F. , Lalande M.
• Języki publikacji: EN

Abstrakty

EN

Adsorption of bovine serum albumin (BSA) at different pHs on carbon supported microfiltration (MF) inorganic membranes was measured by using breakthrough (BT) curves derived from liquid frontal chromatography. Adsorption was quantified in the presence of permeation through the membrane thickness at a constant flow rate. Using the method described, it was confirmed that BSA adsorption is dependent on pH and its maximum is near the BSA isoelectric point ( i.e. pH 4.9). Using Langmuir’s equation, monolayer capacities were determined. It was found that adsorption is of monomolecular type. Analysis of the methods ( called algorithms) used for adsorption calculation was carried out. Monolayer capacities found were generally lower than theoretical BSA monolayer capacity in side-on orientation. It was concluded that such effects as pore blocking, deposition of aggregates inside the membrane or slow formation of dimers were not the main mechanisms of BSA uptake by the MF membranes studied during BT curve formation.

Słowa kluczowe

EN

BSA adsorption; inorganic membranes; breakthrough curve

• Wydawca: Faculty of Chemical Technology and Engineering, University of Technology and Life Sciences, Bydgoszcz
• Czasopismo: Ars Separatoria Acta
• Rocznik: 2002
• Tom: No. 1
• Strony: 111--137
• Opis fizyczny: Bibliogr. 44 poz., rys., tab.

Twórcy

autor

Białopiotrowicz T.

• Maria Curie-Sklodowska University, Department of Physical Chemistry, Maria Curie-Sklodowska Sqr. 3, 20-031 Lublin, Poland

autor

Blanpain P.

• Institut National de la Recherche Agronomique, Laboratoire de Génie des Procédés et Technologie Alimentaires, 369 rue Jules Guesde, BP 39, 59651 Villeneuve d'Ascq Cedex, France

autor

René F.

• Institut National de la Recherche Agronomique, Laboratoire de Génie et Microbiologie des Procédés Alimentaires, CBAI - INA-PG, 78850 Thiverval Grignon, France

autor

Lalande M.

• Institut National de la Recherche Agronomique, Laboratoire de Génie des Procédés et Technologie Alimentaires, 369 rue Jules Guesde, BP 39, 59651 Villeneuve d'Ascq Cedex, France

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