Determination of conjugated fatty acid in ovine milk, meat, fat and intestinal digesta

• Autorzy: Czauderna M. , Kowalczyk J. , Potkański A. , Szumacher-Strabel N.
• Języki publikacji: EN

Abstrakty

EN

Positional and geometric isomers of geometric isomers of linoleic acid (CLA) were separated from interfering species on commercially available two reversed-phase C18-columns (Nova Pak, Waters) in gradient systems composed of acetonitrile and water, utilizing photodiode array detection. The biological samples were hydrolyzed with 2 M NaOH for 35 min at 85°C. After cooling, the hydrolysates were acidified with 4 M HCl and the free fatty acids were extracted with dichloromethane. The CLA isomers were determined directly using UV detection at 234.5 nm or after pre -column derivatization with 2,4’-dibromoacetophenone in the presence of triethylamine and UV detection at 256 and 235 nm. HPLC system with pre-column derivatization enables more efficient fractionation of the CLA isomers than the direct HPLC system. On the other hand, elimination of derivatization procedure provides a less expensive, more specific and simpler analytical tool for determination of CLA than HPLC method with precolumn derivatization. The presented HPLC methods provide analytical tools for simple quantification of CLA in ovine meat, milk, fat and intestinal digesta samples.

Słowa kluczowe

EN

conjugated fatty acid; milk; meat; fat; intestinal digesta; sheep; HPLC

• Wydawca: Faculty of Chemical Technology and Engineering, University of Technology and Life Sciences, Bydgoszcz
• Czasopismo: Ars Separatoria Acta
• Rocznik: 2002
• Tom: No. 1
• Strony: 104--110
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Czauderna M.

• The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

autor

Kowalczyk J.

• The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

autor

Potkański A.

• Department of Animal Nutrition and Feed Management, August Cieszkowski Agricultural University, Wolynska 33, 60-637 Poznan, Poland

autor

Szumacher-Strabel N.

• Department of Animal Nutrition and Feed Management, August Cieszkowski Agricultural University, Wolynska 33, 60-637 Poznan, Poland

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