Simultaneous HPLC-DAD analysis of four methylated flavonols in Amomum koenigii

• Autorzy: Qiao C. F. , Han Q. B. , Song J. Z. , Yiu H. , Xu H. X.
• Języki publikacji: EN

Abstrakty

EN

Four bioactive methylated flavonols isolated from Amomum koenigii J.F. Gmelin, 3,7-dihydroxy-5,3',4'-trimethoxyflavone (1), 5-hydroxy-3,7,4'-trimethoxyflavone (2), 3,7-dihydroxy-5,4'-dimethoxyflavone (3), and 5-hydroxy-3,7,3',4'-tetramethoxyflavone (retusin, 4), have been analyzed by HPLC–DAD on a 4.6 mm × 150 mm, 5-µm particle, C8 column with an acetonitrile–aqueous acetic acid gradient as mobile phase. UV detection was at 360 nm. The method was validated in detail. The results indicated that the total amounts of these four constituents in the seeds were 0.776–0.829%, higher than in the pericarp. Retusin was the major constituent with a maximum content of approximately 0.50% in the seeds. This method is useful for quality control of the crude drug.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2007
• Tom: No. 19
• Strony: 217--226
• Opis fizyczny: Bibliogr. 15 poz., rys., tab.

Twórcy

autor

Qiao C. F.

autor

Han Q. B.

autor

Song J. Z.

autor

Yiu H.

autor

Xu H. X.

• Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, China

Bibliografia

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