Development and validation of an HPTLC method for determination of minocycline in human plasma

• Autorzy: Jain G. K. , Jain N. , Iqbal Z. , Talegaonkar S. , Ahmad F. J. , Khar R. K.
• Języki publikacji: EN

Abstrakty

EN

A new high-performance thin-layer chromatographic (HPTLC) method has been established for determination of minocycline in human plasma. Chromatography was performed on aluminium plates coated with silica gel 60F254; the mobile phase was methanol–acetonitrile–isopropanol–water 5:4:0.5:0.5 (v/v). Densitometric analysis was performed at 345 nm. The method is rapid (single-step extraction with methanol), sensitive (limit of quantification 15.4 ng per zone), precise (CV ≤ 4.61 %), accurate (drug recovery 95.08–100.6%), and linear over the range 100–1200 ng per zone. Recovery of minocycline from plasma samples was 95.8 ± 4.5%. The halflife of minocycline in plasma was 9.9 h at 4°C and 6.3 h at 20°C. Minocycline is stable in human plasma for at least two months at -20°C and can tolerate two freeze–thaw cycles with losses <10%. The method was successfully used to determine therapeutic levels of minocycline.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2007
• Tom: No. 19
• Strony: 197--205
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Jain G. K.

autor

Jain N.

autor

Iqbal Z.

autor

Talegaonkar S.

autor

Ahmad F. J.

autor

Khar R. K.

• Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062, India

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