Development of a reversed-phase HPLC method for analysis of fluocinolone acetonide in gel and ointment

• Autorzy: Chmielewska A. , Konieczna L. , Lamparczyk H.
• Języki publikacji: EN

Abstrakty

EN

An accurate and precise HPLC assay has been established for simul-taneous determination of fluocinolone acetonide and additives in gel. Drugs were chromatographed on a C18 reversed-phase column with 55:45 (v/v) methanol–water as mobile phase and detection at 238 nm. Solution con-centrations were measured on a weight basis to avoid the use of an internal standard. The method was statistically validated for linearity, accuracy, precision, and selectivity. Linearity for assay of fluocinolone acetonide, methyl 4-hydroxybenzoate (nipagin M), and propyl 4-hydroxybenzoate (nipagin P) were confirmed over the ranges 0.5-30, 5-200, and 10-120 µg mL-1, respectively. Because of the simplicity and accuracy of the method, it was used for routine analysis of fluocinolone acetonide in ointment. It does not require any specific sample preparation.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2006
• Tom: No. 16
• Strony: 80--91
• Opis fizyczny: Bibliogr. 20 poz., rys., tab.

Twórcy

autor

Chmielewska A.

autor

Konieczna L.

autor

Lamparczyk H.

• Medical University of Gdańsk, Faculty of Pharmacy, Hallera 107, PL-80-416 Gdańsk, Poland

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