Quantitative determination of pentoxifylline in human plasma

• Autorzy: Chmielewska A. , Konieczna L. , Plenis A. , Lamparczyk H.
• Języki publikacji: EN

Abstrakty

EN

A rapid, optimized, and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for deter-mination of pentoxifylline in human plasma. The analyte was extracted from the plasma with dichloromethane after addition of 0.2 mL 1 M NaOH. HPLC separation was performed on a C18 analytical column (250 mm × 4 mm i.d.) with acetonitrile–water, 45:55 (v/v), as mobile phase. Spectro-photometric detection was performed at 275 nm. Calibration graphs were linear from 25 to 1000 ng mL-1 pentoxifylline. Recovery of the drug from human plasma was 92.1%, and the detection limit was 20 ng mL-1.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2006
• Tom: No. 16
• Strony: 70--79
• Opis fizyczny: Bibliogr. 15 poz., rys., tab.

Twórcy

autor

Chmielewska A.

autor

Konieczna L.

autor

Plenis A.

autor

Lamparczyk H.

• Medical University of Gdańsk, Faculty of Pharmacy, Hallera 107, PL-80-416 Gdańsk, Poland

Bibliografia

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