Analysis of sugars by micellar liquid chromatography with UV detection

• Autorzy: Momenbeik F. , Khorasani J. H.
• Języki publikacji: EN

Abstrakty

EN

A micellar liquid chromatographic procedure is described for si-multaneous determination of sugars previously derivatized with 4-amino-benzoic acid ethyl ester (4-ABEE). The optimized conditions, determined by use of the super-modified simplex method, were temperature 30°C, mo-bile phase containing 17.3 mM sodium dodecyl sulfate, 0.01 M phosphate buffer, pH 6.69, and 7.73% (v/v) ethanol, and UV detection at 307 nm. The 4-ABEE derivatives were separated in 10 min but a peak assigned to excess reagent appears at 13 min. Analytical data including linearity (r > 0.9987), limit of detection (35.47–81.74 µg mL-1), precision (RSD < 5.73%), and accuracy, measured by determination of recovery (>91%), support the use-fulness of the proposed method for measuring sugars. When the proposed method was applied to the analysis of sugars in an infant formula and a sample of sorghum syrup the results were in good agreement with the con-tent reported by manufacturer and with results obtained by refractive index detection.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2006
• Tom: No. 16
• Strony: 58--69
• Opis fizyczny: Bibliogr. 25 poz., rys., tab.

Twórcy

autor

Momenbeik F.

autor

Khorasani J. H.

• Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Iran

Bibliografia

• [1] M. Peris-Tortajada in: L.M.L. Nollet (ed.) Handbook of Food Analysis, Marcel Dekker, New York, 1996; pp. 533–550
• [2] N. Lamari, R. Kuhn, and N.K. Karamanos, J. Chromatogr. B, 793, 15 (2003)
• [3] J.C.M. Waterval, H. Lingeman, A. Bult, and W.J.M. Underberg, Electrophoresis, 21, 4029 (2000)
• [4] H. Kwon and J. Kim, J. Liquid Chromatogr., 7, 1437 (1995)
• [5] T. Akiyama, J. Chromatogr., 588, 53 (1991)
• [6] S. Yasuno, T. Murata, K. Kukubo, T. Yamaguchi, and M. Kamei, Biosci. Biotechnol. Biochem., 61, 1944 (1997)
• [7] H. Kwon and J. Kim, Anal. Biochem., 215, 243 (1993)
• [8] D.B. Gomis, D.M. Tamayo, and J.M. Alonso, Anal. Chim. Acta, 436, 173 (2001)
• [9] A. Berthod and M.C. Garcia Alvarez-Coque, Micellar Liquid Chromatography, Marcel Dekker, New York, 2000
• [10] J.K Strasters, S.T. Kim, and M.G. Khaledi, J. Chromatogr., 586, 221 (1991)
• [11] J.R. Torres-Lapasio, R.M. Villanueva-Camanas, J.M. Sanchis-Mallols, M.J. Medina-Hernandez, and M.C. Garcia-Alvarez-Coque, J. Chromatogr. A, 677, 239 (1994)
• [12] M.D. Rukhadze, G.S. Bezarashvili, M.V. Sebiskveradze, and V.R. Meyer, J. Chromatogr. A, 805, 45 (1998)
• [13] S.L. Morgan and S.N. Deming, J. Chromatogr., 112, 267 (1975)
• [14] S.N. Deming and S.L. Morgan, Anal. Chem., 45, 278A (1973)
• [15] J.A. Nelder and R. Mead, Computer J., 7, 308 (1965)
• [16] S. Srijaranai, R. Burakham, T. Khammeng, and R.L. Deming, Anal. Bioanal. Chem., 374, 145 (2002)
• [17] M.W. Routh, P.A. Swartz, and M.B. Denton, Anal. Chem., 49, 1422 (1977)
• [18] W.T. Wang, N.C. Ledonne, B. Ackerman, and C.H.C. Sweeley, Anal. Biochem., 141, 366 (1984)
• [19] V.R. Meyer, Practical High Performance Liquid Chromatography, Wiley, Chichester, 2000; pp. 45-47
• [20] J.P. Foley and J.G. Dorsey, Anal. Chem., 55, 730 (1983)
• [21] M.F. Borgerding, F.H. Quina, W.L. Hinze, J. Bowermaster, and H.M. McNair, Anal. Chem., 60, 2520 (1988)
• [22] M.G. Khaledi, Anal. Chem., 60, 876 (1988)
• [23] M.G. Khaledi, J.K. Strasters, A.H. Rodgers, and E.D. Breyer, Anal. Chem., 62, 130 (1990)
• [24] J.C. Berridge and E.G. Morrissey, J. Chromatogr., 316, 69 (1984)
• [25] G. Kuepper, Sweet Sorghum Production and Processing, The Kerr Center, Poteau, OK, 1992