Determination of seven water-soluble vitamins in tarhana, a traditional Turkish cereal food, by high-performance liquid chromatography

• Autorzy: Ekinci R. , Kadakal Ç.
• Języki publikacji: EN

Abstrakty

EN

A reversed-phase high-performance liquid chromatographic proce-dure has been developed for determination of water-soluble vitamins (as-corbic acid, niacin, panthothenic acid (vitamin B5), pyridoxine (vitamin B6), thiamine (vitamin B1), folic acid, and riboflavin (vitamin B2)) in tar-hana samples, a traditional Turkish cereal food. Sample-treatment entails SPE with Sep-Pak C18 (500-mg) cartridges. The water-soluble vitamins were analyzed by HPLC on a Discovery C-18 150 mm × 4.6 mm column with 0.1 mol L-1 KH2PO4 (pH 7.0)–methanol, 90:10, as mobile phase (0.7 mL min-1) in isocratic mode. Identification of compounds was achieved by comparing their retention times and UV spectra with those of standards stored in a data bank. The detection limits ranged from 0.1 to 0.5 mg L-1. The accuracy of the method was tested by measuring average recovery; values ranged between 96.51 and 99.40%.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2005
• Tom: No. 15
• Strony: 289--297
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Ekinci R.

• Pamukkale University, College of Engineering, Department of Food Engineering, Çamlýk, Denizli, 20020, Turkey

autor

Kadakal Ç.

• Pamukkale University, College of Engineering, Department of Food Engineering, Çamlýk, Denizli, 20020, Turkey

Bibliografia

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