HPLC separation of linezolid enantiomers using polysaccharide-based chiral stationary phases

• Autorzy: Bielejewska A. , Duszczyk K. , Żukowski J.
• Języki publikacji: EN

Abstrakty

EN

Separation of the enantiomers of linezolid has been compared on derivatized cellulose and amylose chiral stationary phases (Chiracel OD and Chiralpak AD) with mixtures of hexane with 1-propanol, 2-propanol, or ethanol as mobile phases. It was found that use of a small amount of water as mobile phase additive can improve or prevent enantioseparation, depending on the column. The order of elution of the linezolid enantiomers was different on the Chiracel OD and Chiralpak AD columns, and baseline resolution was achieved on the Chiralpak AD column only. Reversal of the order of elution of the enantiomers on changing from the propanols to ethanol was observed on both columns. The effect of temperature (in the narrow range 15–35°C) on retention and enantioselectivity was studied for both columns and a variety of mobile phases. The standard enthalpy (ΔH°) and entropy (ΔS°) changes for solute transfer between the mobile and sta-tionary phases were also estimated.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2005
• Tom: No. 15
• Strony: 183--191
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Bielejewska A.

• Institute of Physical Chemistry PAS, Kasprzaka 44/52, 01-224 Warsaw, Poland
• Pharmaceutical Research Institute, Rydygiera 8, 02-187 Warsaw, Poland

autor

Duszczyk K.

• Institute of Physical Chemistry PAS, Kasprzaka 44/52, 01-224 Warsaw, Poland

autor

Żukowski J.

• GlaxoSmithKline, Analyt Chem, Harlow CM19 5AD, Essex, England

Bibliografia

• [1] S.M. Swaney, H. Aoki, M.C. Ganoza, and D.L. Shinabarger, Antimicrob. Agents Chemother., 42, 3251 (1998)
• [2] M. Ehrlich, R. Trittler, F.D. Daschner, and K. Kummerer, J. Chromatogr. B, 755, 373 (2001)
• [3] Y. Okamoto and Y. Kaida, J. Chromatogr. A, 666, 403 (1994)
• [4] Y. Okamoto and E. Yashima, Angew. Chem., Int. Ed. Eng., 73, 1020 (1998)
• [5] M. Okamoto, J. Pharm. Biomed. Anal., 27, 401 (2003)
• [6] T Wang and Y.W. Chen, J. Chromatogr. A, 855, 411 (1999)
• [7] T Wang, Y.W. Chen, and A. Vailaya, J. Chromatogr. A, 902, 345 (2000)
• [8] J. Dingenen, Polysaccharide Phases in Enantioseparations, in: G Subramanian (ed.). A Practical Approach to Chiral Separation by Liquid Chromatography, VCH, Weinheim, 1994, p. 115
• [9] J.G. Ning, Enantiomer, 5, 323 (2000)
• [10] K. Balmer, P.O. Lagerstrom, B.A. Persson, and G. Schill, J. Chromatogr., 592, 331 (1992)
• [11] C. Narayana, T. Suresh, S.M. Rao, P.K. Dubey, and J.M. Babu, J. Pharm. Biomed. Anal., 32, 21 (2003)
• [12] H. Koller, K.H. Rimbock, and A. Mannschreck, J. Chromatogr., 282, 89 (1983)
• [13] J. Chmielowiec and H. Sawitzky, J. Chromatogr. Sci., 17, 245 (1979)