Densitometric acquisition of concentration profiles in planar chromatography and its possible shortcomings. Part 1. 4-phenylbutyric acid as analyte

• Autorzy: Sajewicz M. , Pieniak A. , Piętka R. , Kaczmarski K. , Kowalska T.
• Języki publikacji: EN

Abstrakty

EN

In this paper we describe part of a study originally devoted to inve-stigating lateral interactions of 4-phenylbutyric acid in 'mild' chromatogra-phic systems comprising low-activity stationary phases (microcrystalline cellulose or chromatography paper) and low-polarity decalin as mobile phase. The presence or absence of lateral interactions of an analyte in a given chromatographic system can be judged from the shape of the concentration profile and for this purpose densitograms of the bands of interest must first be acquired. Our attention was unexpectedly attracted by a largely surprising observation that the concentration profile of 4-phe-nylbutyric acid on cellulose powder could be measured easily on a freshly developed chromatogram only after drying whereas on chromatography paper developed at room temperature it was hardly detectable, and became fully shaped only two days later. To explain this striking phenomenon we had to perform several additional experiments and, although the final answer is not fully conclusive, a tentative explanation can already be presented.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2004
• Tom: No. 14
• Strony: 5--15
• Opis fizyczny: Bibliogr. 6 poz., rys.

Twórcy

autor

Sajewicz M.

• Institute of Chemistry, Silesian University, 9, Szkolna Street, 40-006 Katowice, Poland

autor

Pieniak A.

• Institute of Chemistry, Silesian University, 9, Szkolna Street, 40-006 Katowice, Poland

autor

Piętka R.

• Institute of Chemistry, Silesian University, 9, Szkolna Street, 40-006 Katowice, Poland

autor

Kaczmarski K.

• Faculty of Chemistry, Technical University of Rzeszów, Rzeszów, Poland

autor

Kowalska T.

• Institute of Chemistry, Silesian University, 9, Szkolna Street, 40-006 Katowice, Poland

Bibliografia

• [1] R.H. Fowler and E.A. Guggenheim, Statistical Thermodynamics, Cambridge University Press, Cambridge, UK, 1960
• [2] I. Quinones and G. Guiochon, J. Chromatogr. A, 796, 15 (1998)
• [3] G.I. Berezin and A.V. Kiselev, J. Colloid Interface Sci., 38, 227 (1972)
• [4] G.I. Berezin, A.V. Kiselev, and R.T. Sagatelyan, J. Colloid Interface Sci., 38, 335 (1972)
• [5] W. Prus, K. Kaczmarski, K. Tyrpień, M. Borys, and T. Kowalska, J. Liq. Chromatogr. Related Technol., 24, 1381 (2001)
• [6] K. Kaczmarski, W. Prus, C. Dobosz, P. Bojda, and T. Kowalska, J. Liq. Chromatogr. Related Technol., 25, 1469 (2002)