Computer optimization of the rp hplc separation of some taxoids from yew extracts

• Autorzy: Hajnos M. Ł. , Waksmundzka-Hajnos M. , Głowniak K.
• Języki publikacji: EN

Abstrakty

EN

Drylab G software has been used to optimize the reversed-phase HPLC separation of taxoids and co-extracted substances from yew. Two preliminary runs based on a linear gradient from 5 to 100% acetonitrile (ACN) in 20 or 60 min were shown to be satisfactory for optimization of resolution. The optimization experiments were performed on a purified extract fortified with taxoid standards. The identity and purity of the peaks were verified by use of photodiode-array detection. Agreement between simulated and experimental data was good. The optimized chromatographic system can be used for quantitative analysis of paclitaxel, cephalomannine, and 10-deacetylbaccatin in yew extracts.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2002
• Tom: No. 12
• Strony: 211--218
• Opis fizyczny: Bibliogr. 26 poz., rys., tab.

Twórcy

autor

Hajnos M. Ł.

• Department of Pharmacognosy, Medical University, Peowiaków 12, 20-007 Lublin, Poland

autor

Waksmundzka-Hajnos M.

• Department of Inorganic and Analytical Chemistry, Medical University, Staszica 6, 20-081 Lublin, Poland

autor

Głowniak K.

• Department of Pharmacognosy, Medical University, Peowiaków 12, 20-007 Lublin, Poland

Bibliografia

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