Thin layer chromatographic analysis of carbohydrates and amino acids in Schistosoma mansoni (Trematoda) cercariae

• Autorzy: Wagner S. D. , Pachuski J. , Fried B. , Sherma J.
• Języki publikacji: EN

Abstrakty

EN

Thin layer chromatography (TLC) has been used to analyze the carbohydrates and amino acids present in the cercariae of Schistosoma mansoni released from the gastropod Biomphalaria glabrata. Visual observation of the carbohydrate chromatograms showed the presence of glucose and raffinose and three unidentified ?-naphthol-positive zones. Quantification by densitometry of glucose zones in three cercarial samples gave a mean value of 0.491 ng cercaria-1. Raffinose was found to be present at a level of 0.0250 ng cercaria-1 in one sample and below the 0.0200 ng cercaria-1 limit of quantification in two others. Visual observation of the amino acid chromatograms confirmed the presence of histidine, tryptophan, isoleucine, alanine, and proline. Several other unknown ninhydrin-positive zones were also detected. Quantification of the histidine and tryptophan zones in cercarial samples by densitometry gave mean values of 3.71 and 0.588 ng cercaria-1, respectively. The possible functions of carbohydrates and amino acids in the cercariae of S. mansoni are discussed.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2002
• Tom: No. 12
• Strony: 159--169
• Opis fizyczny: Bibliogr. 12 poz., rys., tab.

Twórcy

autor

Wagner S. D.

• Department of Chemistry, Lafayette College, Easton, Pennsylvania 18042, USA

autor

Pachuski J.

• Department of Chemistry, Lafayette College, Easton, Pennsylvania 18042, USA

autor

Fried B.

• Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA

autor

Sherma J.

• Department of Chemistry, Lafayette College, Easton, Pennsylvania 18042, USA

Bibliografia

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