Analysis of the caffeine in alertness tablets and caplets by high-performance thin-layer chromatography with ultraviolet absorption densitometry of fluorescence-quenched zones

• Autorzy: Ruddy D. , Sherma J.
• Języki publikacji: EN

Abstrakty

EN

A quantitative method using silica gel HPTLC plates with fluorescent indicator, automated sample application, and UV absorption densitometry has been developed for the determination of caffeine in pharmaceutical preparations designed to promote alertness. Tablet, coated tablet, and coated caplet products containing caffeine as the active ingredient were analyzed to test the applicability of the new method. Precision was evaluated by replicate analysis of samples and accuracy by analysis of spiked blank samples containing inactive ingredients in common with the caffeine products. The amount of caffeine in the preparations analyzed ranged from 101 to 121% of label values. Precision ranged from 1.8 to 2.3% relative standard deviation, and the errors from three spiked blank analyses averaged 0.983% compared with fortification levels. The limit of detection was 200 ng caffeine.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2002
• Tom: No. 12
• Strony: 143--150
• Opis fizyczny: Bibliogr. 8 poz., rys., tab.

Twórcy

autor

Ruddy D.

• Department of Chemistry, Lafayette College, Easton, PA 18042, USA

autor

Sherma J.

• Department of Chemistry, Lafayette College, Easton, PA 18042, USA

Bibliografia

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